中国烟草科学2025，Vol.46Issue(2)：45-51,7.DOI:10.13496/j.issn.1007-5119.2025.02.006
长沙烟区烟草赤星病病害调查与病原菌鉴定
Investigation of Tobacco Brown Spot Disease and Pathogen Identification in Changsha Tobacco-Growing Region
摘要
Abstract
Brown spot disease is one of the primary factors affecting safety of leaf tobacco quality in Changsha tobacco-growing region.To investigate the occurrence and identify the pathogenic species causing tobacco brown spot disease,a comprehensive survey was conducted on incidence rate and disease index of brown spot disease across the tobacco production towns in Changsha.Results showed that the incidence rate and disease index ranged 0.56%-16.67%and 0.06-5.43 respectively.Through combined morphological and molecular biological identification of isolated pathogens,the study revealed that the causative agents were Alternaria alternata and A.tenuissima,with the detection rate of A.tenuissima up to 75.00%.Further in this study,rDNA-ITS gene was confirmed to be excessive conservation for species-level differentiation within the Alternaria genus,whereas the plasma membrane ATPase gene displayed concordance with morphological identification results,proving suitable for interspecies discrimination.In conclusion,the pathogens of tobacco brown spot disease in Changsha tobacco-growing region exhibit high diversity,with Alternaria alternata being predominant.关键词
长沙烟区/烟草赤星病/病原菌鉴定/链格孢/细极链格孢Key words
Changsha tobacco-growing region/tobacco brown spot disease/pathogen identification/A.alternata/A.tenuissima分类
植物保护学引用本文复制引用
谢林,陈武,蔡海林,李建勇,翟争光,邹硕,何昊欣,付竞,黄彬彬,刘清术..长沙烟区烟草赤星病病害调查与病原菌鉴定[J].中国烟草科学,2025,46(2):45-51,7.基金项目
中国烟草总公司湖南省公司科技项目(HN2024KJ04) （HN2024KJ04）
湖南省烟草公司长沙市公司科技项目(CS2022KJ02) （CS2022KJ02）
