中国烟草科学2025，Vol.46Issue(2)：83-92,112,11.DOI:10.13496/j.issn.1007-5119.2025.02.011
烟草SRS基因家族鉴定与非生物胁迫诱导表达分析
Identification of Tobacco SRS Gene Family and Abiotic Stress-induced Expression Analysis
摘要
Abstract
The SHI-related sequence(SRS)gene family encodes a class of plant-specific zinc finger protein transcription factors that play crucial roles in regulating plant growth,development and stress responses.To explore the function of the SRS gene family in tobacco,18 NtSRS genes were identified from the tobacco genome database and designated as NtSRS1 to NtSRS18.Bioinformatics analyses were conducted to investigate their protein characteristics,subcellular localization,phylogenetic relationships,gene structures,and promoter elements.Additionally,expression patterns of NtSRS family across different tissues and organs,as well as their responses to drought,salt,and low temperature stress,were analyzed using qRT-PCR.Results revealed that NtSRS proteins contained the conserved DUF702 domain,with most localized in the nucleus.Phylogenetic analysis clustered the NtSRS family into 5 subfamilies,with members of the same subfamily exhibiting highly conserved gene and protein structures.Promoter analysis identified numerous hormone-responsive elements and stress-responsive elements within the regulatory regions of NtSRS genes.Expression profiling showed that NtSRS genes exhibited significant tissue-specific expression,with most highly expressed in flower buds and stems.Notably,NtSRS3 and NtSRS16 were strongly induced under drought,salt,and low temperature stress conditions.Overall,this study provides a comprehensive characterization of the tobacco SRS gene family,offering a theoretical basis for the genetic improvement of tobacco germplasm resources.关键词
烟草/SRS基因家族/非生物胁迫/表达模式分析Key words
tobacco/SRS gene family/abiotic stress/expression pattern analysis分类
农业科技引用本文复制引用
杨亚仑,邓智超,董若男,潘晓璐,吴荣荣,刘涛,高晓明..烟草SRS基因家族鉴定与非生物胁迫诱导表达分析[J].中国烟草科学,2025,46(2):83-92,112,11.基金项目
中国农业科学院科技创新工程项目(ASTIP-TRIC02) （ASTIP-TRIC02）
