北华大学学报（自然科学版）2025，Vol.26Issue(3)：335-339,5.DOI:10.11713/j.issn.1009-4822.2025.03.009
幽门螺杆菌阳性的慢性萎缩性胃炎患者胃黏膜组织miR-34a和FOXD3表达特点
Expression Characteristics of miR-34a and FOXD3 in Gastric Mucosal Tissue of Helicobacter Pylori Positive Chronic Atrophic Gastritis Patients
摘要
Abstract
Objective To investigate the expression characteristics and clinical significance of miR-34a and forkhead box D3(FOXD3)in gastric mucosal tissues of Helicobacter pylori(Hp)positive chronic atrophic gastritis patients.Methods Ninety-two Hp positive chronic atrophic gastritis patients were studied,and atrophic gastric mucosal tissues(diseased gastric mucosal tissues)as well as peripheral normal gastric mucosal tissues(normal gastric mucosal tissues)were collected at the time of gastroscopy.miR-34a and FOXD3 mRNA expression levels were detected in tissue specimens by RT-PCR,and the protein expression level of FOXD3 was assessed in tissue specimens by Western blot.The patients were followed up for 1 a,and their prognosis was recorded.ROC curves were constructed to analyze the predictive value of miR-34a and FOXD3 expression on the patients'prognosis.Results The expression level of miR-34a was lower in atrophic gastric mucosal tissues of patients with Hp positive chronic atrophic gastritis compared to normal gastric mucosal tissues(P＜0.01).In contrast,the expression levels of FOXD3 mRNA and FOXD3 protein were higher in atrophic gastric mucosal tissues than in normal gastric mucosal tissues(P＜0.05).Among patients,those with severe gastritis exhibited lower miR-34a levels than those with moderate and mild gastritis,while FOXD3 mRNA and protein levels were higher in severe group(P＜0.05).Additionally,the miR-34a expression level in moderate patients was lower than in mild patients,and the FOXD3 mRNA and protein levels were higher in moderate patients compared to mild patients(P＜0.05).In atrophic gastric mucosal tissues of chronic atrophic gastritis patients with poor prognosis,miR-34a levels were lower,and FOXD3 mRNA and protein expression levels were higher than those in patients with good prognosis(all P＜0.05).The severity of disease in patients with chronic atrophic gastritis was negatively correlated with miR-34a expression in atrophic gastric mucosal tissue(P＜0.05)and positively correlated with FOXD3 mRNA and FOXD3 protein expression(P＜0.05).Additionally,the prognostic level was positively correlated with miR-34a expression in atrophic gastric mucosal tissue(P＜0.05)and negatively correlated with FOXD3 mRNA and FOXD3 protein expression(P＜0.05).The combination of miR-34a,FOXD3 mRNA and FOXD3 protein demonstrated the highest predictive value(AUC=0.953).Conclusion Low expression of miR-34a and high expression of FOXD3 in gastric mucosall tissues of Hp positive chronic atrophic gastritis patients are correlated with the severity of the disease and prognosis,and their combination has a high predictive value for patient prognosis.关键词
慢性萎缩性胃炎/Hp阳性/miR-34a/叉头框转录因子D3/预后Key words
chronic atrophic gastritis/Hp positive/miR-34a/forkhead box D3/prognosis分类
医药卫生引用本文复制引用
贾纯增,陈锐,马雨杰,王玘..幽门螺杆菌阳性的慢性萎缩性胃炎患者胃黏膜组织miR-34a和FOXD3表达特点[J].北华大学学报（自然科学版）,2025,26(3):335-339,5.基金项目
北京市卫生健康科技成果和适宜技术推广项目(BHTPP2022035). （BHTPP2022035）
