北华大学学报（自然科学版）2025，Vol.26Issue(3)：317-321,5.DOI:10.11713/j.issn.1009-4822.2025.03.006
五味子-黄芪混合多糖对D-半乳糖诱导小鼠肝损伤的保护作用
Protective Effects of Polysaccharide from Mixed Schisandra chinensis and Astragali Radix on D-galactose-induced Liver Injury in Mice
摘要
Abstract
Objective To investigate the protective effect and possible mechanism of action of polysaccharide from mixed Schisandra chinensis and Astragali Radix(SAP)against D-galactose-induced liver injury in mice.Methods KM mice were randomly divided into five groups:control group,model group,and SAP low(75 mg/kg),medium(150 mg/kg)and high(300 mg/kg)dose.The back of neck was injected subcutaneously with D-galactose(500 mg/kg)to establish a mouse liver injury model.During the test period,the general state of mice was recorded,the liver index was measured,and levels of ALT and AST in serum,and T-SOD activity and MDA content in both serum and liver were detected.Hematoxylin-eosin staining was used to observe pathological changes in the liver.Results Compared with control group,the liver index and serum levels of ALT and AST in model group were significantly higher(P＜0.05,P＜0.01).The T-SOD activity in both serum and liver was reduced,and the MDA content was elevated(P＜0.01,P＜0.05).Hematoxylin-eosin staining showed that the arrangement of hepatocytes was disorganized,with a marked increase in cell volume,and that the cytoplasm was sparsely and faintly stained,indicating hydropic degeneration.Compared with model group,the liver index and serum levels of ALT and AST in mice from high dose group of SAP were significantly reduced(P＜0.05,P＜0.01).T-SOD activity in serum and liver rebounded to varying degrees,and the MDA content was significantly reduced(P＜0.01,P＜0.05).Hematoxylin-eosin staining indicated that the degree of hepatic pathological damage was significantly reduced in each treated group,with hepatocytes arranged more neatly.Additionally,the swelling,deformation and necrosis of hepatocytes were reduced,and watery degeneration was improved.Conclusion SAP has a better protective effect on D-galactose-induced liver injury.关键词
五味子/黄芪/D-半乳糖/肝损伤/抗氧化Key words
Schisandra chinensis/Astragali Radix/D-galactose/liver injury/antioxidant分类
医药卫生引用本文复制引用
李婷,邹一卓,杨茜茹,陈小雪,孙毓蔓,孙靖辉,李贺,王春梅..五味子-黄芪混合多糖对D-半乳糖诱导小鼠肝损伤的保护作用[J].北华大学学报（自然科学版）,2025,26(3):317-321,5.基金项目
吉林省自然科学基金项目(YDZJ202401018ZYTS) （YDZJ202401018ZYTS）
吉林省预算内基本建设资金项目(2023C037-9) （2023C037-9）
北华大学研究生创新计划项目(2023027). （2023027）
