北华大学学报（自然科学版）2025，Vol.26Issue(3)：322-328,7.DOI:10.11713/j.issn.1009-4822.2025.03.007
基于TLR4/NF-κB/NLRP3信号通路的马齿苋生物碱对溃疡性结肠炎作用机制
Mechanism of Purslane Alkaloids on Ulcerative Colitis Based on TLR4/NF-κB/NLRP3 Signalling Pathway
摘要
Abstract
Objective To investigate therapeutic effects and regulatory mechanisms of purslane alkaloids(PA)on ulcerative colitis(UC)in mice.Methods Thirty-five mice were randomly divided into control group,model group and drug treatment group(which included purslane alkaloids groups at low,medium and high doses,mesalazine group,as well as purslane alkaloids combined with mesalazine group).The UC animal model was constructed by providing pure water to the mice in control group and 3％dextran sulfate sodium salt(DSS)solution to the mice in model and drug treatment groups for the first 7 days.From day 8 onward,saline was administered to control and model groups,while the mice in drug treatment group began receiving fixed-dose drug gavage treatment for 14 days.During this period,the health status,body mass,fecal characteristics and the presence or absence of bloody stools were recorded daily.At the end of experiment,the disease activity index(DAI)was calculated for each group of mice,and the length of colon was measured.Hematoxylin-eosin staining was used to observe histopathological changes in the colon.Enzyme-linked immunosorbent assay(ELISA)was used to measure the levels of pro-inflammatory factors interleukin-1β(IL-1β),interleukin-6(IL-6)and tumour necrosis factor-α(TNF-α)in serum and colon tissues.The protein and mRNA exprossion of Toll-like receptor 4(TLR4),nuclear factor kappa-B(NF-κB)and Nod-like receptor3(NLRP3)of colon tissues were measured by Western blot and real-time fluorescence quantitative PCR(RT-qPCR).Results Compared with model group,purslane alkaloids treatment group and combined group could alleviate symptoms and gain body mass in mice(P＜0.05,P＜0.01),reduce DAI score(P＜0.05,P＜0.01),alleviate shortening of colon tissues(P＜0.01)and pathological damage,and reduce the levels of inflammation of IL-1β,IL-6 and TNF-α in the serum and colon tissues of mice(P＜0.05,P＜0.01).Additionally,there was a significant down-regulation of the protein and mRNA expression levels of TLR4,NF-κB and NLRP3 in colon tissues(P＜0.05,P＜0.01).Conclusion Purslane alkaloids have significant therapeutic effects on acute UC in mice,as they can reduce the levels of pro-inflammatory cytokines in colon tissues of UC mice and significantly alleviate pathological damage.Their anti-inflammatory mechanism may be related to the inhibition of the TLR4/NF-κB/NLRP3 signaling pathway.关键词
马齿苋生物碱/溃疡性结肠炎/Toll样受体4/核因子kappa-B/Nod样受体蛋白3Key words
purslane alkaloids/ulcerative colitis/Toll-like receptor 4/nuclear factor kappa-B/Nod-like receptor3分类
医药卫生引用本文复制引用
宋英英,郑佳慧,艾金霞,杨志平..基于TLR4/NF-κB/NLRP3信号通路的马齿苋生物碱对溃疡性结肠炎作用机制[J].北华大学学报（自然科学版）,2025,26(3):322-328,7.基金项目
吉林省科技发展计划项目(YDZJ202301ZYTS507) （YDZJ202301ZYTS507）
北华大学研究生创新计划项目(2023080) （2023080）
北华大学医学技术学院"迈瑞"学生创新项目(20210010). （20210010）
