吉林大学学报（医学版）2025，Vol.51Issue(2)：325-332,8.DOI:10.13481/j.1671-587X.20250206
小鼠骨髓间充质干细胞通过JAK2/STAT3信号通路对成纤维细胞增殖和胶原表达水平的影响
Effect of bone marrow mesenchymal stem cells of mice on proliferation and collagen expression levels of fibroblasts through JAK2/STAT3 signaling pathway
摘要
Abstract
Objective:To investigate the effect of bone marrow mesenchymal stem cells(BMSCs)on the proliferation and collagen expression levels of L929 cells,and to clarify its related mechanism.Methods:The BMSCs were extracted from the 4-week-old C57BL/6 mice.The phenotypes of BMSCs were identified by immunofluorescence staining.The L929 cells were divided into control group(L929 cells),co-culture group(L929 cells and BMSCs),inhibitor of Janus kinase(JAK)WP1066 group(WP1066-treated L929 cells and BMSCs),and dimethyl sulfoxide(DMSO)group(DMSO-treated L929 cells and BMSCs).Cell counting kit-8(CCK-8)assay was used to detect the proliferation activities of the L929 cells in various groups at different time points;Western blotting method was used to detect the expression levels of type Ⅰ collagen(Col Ⅰ)and type Ⅲ collagen(Col Ⅲ)in the L929 cells in various groups;immunofluorescence staining was used to detect the expressions of Col Ⅰ and Col Ⅲ proteins in the L929 cells in various groups.Results:The fluorescence assay results of surface antigen(SA)showed that the surface markers CD29+,CD45-,CD90+and CD105+were found in the BMSCs.The CCK-8 assay results showed that compared with control group,the proliferation activities of the L929 cells in co-culture group and DMSO group were significantly increased(P＜0.01);compared with co-culture group,the proliferation activity of the L929 cells in WP1066 group was significantly decreased(P＜0.01).The Western blotting method results showed that compared with control group,the expression levels of Col Ⅰ and Col Ⅲ proteins in the L929 cells in co-culture group and DMSO group were significantly increased(P＜0.01);compared with co-culture group,the expression levels of Col Ⅰ and Col Ⅲ proteins in the L929 cells in WP1066 group were significantly decreased(P＜0.01);compared with DMSO group,the expression levels of Col Ⅰ and Col Ⅲ proteins in the L929 cells in WP1066 group were significantly decreased(P＜0.01).The immunofluorescence staining results showed that compared with control group,the fluorescence intensities of Col Ⅰ and Col Ⅲ proteins in the L929 cells in co-culture group and DMSO group were significantly increased(P＜0.01);compared with co-culture group,the fluorescence intensities of Col Ⅰ and Col Ⅲ in the L929 cells in WP1066 group were significantly decreased(P＜0.01);compared with DMSO group,the fluorescence intensities of Col Ⅰ and Col Ⅲ in the L929 cells in WP1066 group were significantly decreased(P＜0.01).Conclusion:MSCs can promote the proliferation and collagen production of the L929 cells of the mice through the JAK2/signal transducer and activator of transcription 3(STAT3)signaling pathway.关键词
盆底功能障碍性疾病/骨髓间充质干细胞/成纤维细胞/Ⅰ型胶原蛋白/Ⅲ型胶原蛋白/Janus激酶2/信号转导和转录激活子3Key words
Pelvic floor dysfunction/Bone marrow mesenchymal stem cell/Fibroblast/Type Ⅰ collagen/Type Ⅲ collagen/Janus kinase 2/Signal transducer and activator of transcription 3分类
临床医学引用本文复制引用
黎涵玥,阳莲,刘剑锋,张舒飞,洪莉..小鼠骨髓间充质干细胞通过JAK2/STAT3信号通路对成纤维细胞增殖和胶原表达水平的影响[J].吉林大学学报（医学版）,2025,51(2):325-332,8.基金项目
国家自然科学基金项目(82371639) （82371639）
国家重点研发计划项目(2021YFC2701300,2021YFC2701302) （2021YFC2701300,2021YFC2701302）
湖北省科技厅自然科学基金项目(2022CFB124) （2022CFB124）
湖北省科技厅重点研发项目(2022BCA045) （2022BCA045）
湖北省领军人才项目([2019]47) （[2019]47）
