吉林大学学报（医学版）2025，Vol.51Issue(2)：403-411,9.DOI:10.13481/j.1671-587X.20250214
高浓度葡萄糖对体外Raw264.7巨噬细胞极化状态的影响
Effect of high glucose on polarization of Raw264.7 macrophages in vitro
摘要
Abstract
Objective:To investigate the induction effect of different concentrations of glucose on the polarization of Raw264.7 macrophages in vitro.Methods:The Raw264.7 cells cultured in DMEM medium were divided into control group(5.5 mmol·L-1 glucose),different high-glucose groups(15.0,25.0,35.0,and 45.0 mmol·L-1 glucose),and positive control group[lipopolysaccharide(LPS)].The cells were cultured for 3,6,and 9 h,respectively.Cell morphology was observed in various groups.Cell counting kit-8(CCK-8)method was used to assess the survival rates of cells in various groups.Real-time fluorescence quantitative PCR(RT-qPCR)method was used to detect the expression levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and interleukin-10(IL-10)mRNA in cells in various groups.Enzyme-linked immunosorbent assay(ELISA)was used to measure the levels of IL-6,TNF-α,and IL-10 in the cell supernatants in various groups.Flow cytometry was used to determine the percentages of M1 and M2 macrophage markers,CD86+and CD163+cells,in various groups.Results:In control group,theRaw264.7 cells adhered to the culture dish,with a predominantly round morphology.The cells in 35.0 mmol·L-1 high-glucose group and positive control group showed elongated shapes and pseudopodium formation,indicating inflammatory changes.Compared with control group,the survival rates in different high-glucose groups at 6,12,24,and 48 h after treatment were increased(P＜0.05).Compared with control group,after 3 h of treatment,the expression levels of IL-6 and IL-10 mRNA in the cells in 35.0 mmol·L-1 high-glucose group were increased(P＜0.05 or P＜0.01),while no significant changes were observed in IL-6,TNF-α,and IL-10 levels in the cell supernatants(P＞0.05);after 6 h of treatment,the expression level of TNF-α mRNA in the cells in 35.0 mmol·L-1 high-glucose group was increased(P＜0.001),and the levels of IL-6,TNF-α,and IL-10 in the cell supernatants were significantly increased(P＜0.05 or P＜0.001);after 3 h of treatment,the percentages of macrophage markers CD86+cells and CD163+cells in 35.0 mmolL-1 high-glucose group were significantly increased(P＜0.01 or P＜0.001).Conclusion:A certain high concentration of glucose may induce the polarization of Raw264.7 macrophages towards the M1 subtype in vitro.关键词
Raw264.7细胞/葡萄糖/炎症/巨噬细胞极化/炎症细胞因子Key words
Raw264.7 cells/Glucose/Inflammation/Macrophage polarization/Inflammatory cytokine分类
医药卫生引用本文复制引用
胡晓霞,李亚龙,杨东亮,拉巴泽仁,刘欣跃..高浓度葡萄糖对体外Raw264.7巨噬细胞极化状态的影响[J].吉林大学学报（医学版）,2025,51(2):403-411,9.基金项目
甘肃省科技厅自然科学基金项目(23JRRA0958) （23JRRA0958）
兰州大学第二医院(第二临床医学院)"萃英科技创新"计划项目(CY2023-MS-B12) （第二临床医学院）
