结直肠肛门外科2025，Vol.31Issue(2)：125-132,8.DOI:10.19668/j.cnki.issn1674-0491.2025.02.007
Tricellulin调控结直肠癌细胞侵袭迁移和EMT发生的机制研究
Mechanistic study on Tricellulin regulation of colorectal cancer cell invasion,metastasis and epithelial-mesenchymal transition
摘要
Abstract
Objectives To investigate the related molecular mechanisms by which Tricellulin regulates the invasion,migra-tion and metastasis and epithelial-mesenchymal transition(EMT)progression in colorectal cancer.Methods Tissue speci-mens from 40 colorectal cancer patients(cancerous and adjacent normal tissues)treated at The Second Affiliated Hospi-tal of Guangxi Medical University between March 2019 and December 2023 were analyzed.Immunohistochemistry as-sessed Tricellulin expression,and its correlation with clinical-pathological features was evaluated.Stable Tricellulin-overexpressing and-knockdown colorectal cancer cell models(HCT116,HT-29)were validated via Western blotting and real-time fluorescence quantitative PCR.The experimental groups included:HCT116 knockdown group(HCT116-KD group)and its negative control(HCT116-NC-group),HCT116 overexpression group(HCT116-OE group)and its negative control(HCT116-NC+group),HCT116 blank control group(wild-type HCT116 cells,designated as HCT116 group);HT-29 knock-down group(HT-29-KD group)and its negative control(HT-29-NC-group),HT-29 overexpression group(HT-29-OE group)and its negative control(HT-29-NC+group),HT-29 blank control group(wild-type HT-29 cells,designated as HT-29 group).Cell migration assays evaluated colorectal cancer cells(HCT116)migratory effects,while Western blotting ana-lyzed EMT markers and TGFβ/Smad pathway proteins.Results The positive expression rate of Tricellulin in colorectal cancer tissues was higher than that in adjacent normal tissues.Among the 40 colorectal cancer cases,Tricellulin was highly expressed in 30 cases(75％)in in cancer tissues and 3 cases(7.5％)in adjacent tissues(P＜0.05).The expression of Tricellulin was not related to patients'gender or age but was associated with lymph node metastasis,distant metasta-sis,and TNM staging in colorectal cancer patients(P＜0.05).The results of real-time fluorescence quantitative PCR and Western blotting showed that the HCT116 and HT-29 cell models with stable low expression/overexpression of Tricellu-lin were successfully established.The number of migrating cells in the HCT116-KD group was less than that in the HCT116-NC-group(P＜0.05),and the number of migrating cells in the HCT116-OE group was higher than that in the HCT116-NC+group(P＜0.05).The relative expression levels of TGFβ1,P-Smad3,and P-Smad2 proteins in the HCT116-NC-and HT-29-KD groups were lower than those in their respective control groups(P＜0.05),while the relative expres-sion levels of TGFβ1,P-Smad3,and P-Smad2 proteins in the HCT116-OE and HT-29-OE groups were higher than those in their respective control groups(P＜0.05).Conclusions Tricellulin promotes colorectal cancer cell invasion,migration,and EMT progression potentially via TGFβ1/Smad signaling pathway activation.关键词
结直肠癌/Tricellulin/侵袭/迁移/上皮间质转化/TGFβ1/Smad信号通路Key words
colorectal cancer/Tricellulin/invasion/migration/epithelial-mesenchymal transition/TGFβ1/Smad signaling pathway分类
临床医学引用本文复制引用
阳文芳,黄杰安,廖存,张金秀,程若溪,潘小萍,陈秋玲,谭炎林,冯考艳,覃蒙斌..Tricellulin调控结直肠癌细胞侵袭迁移和EMT发生的机制研究[J].结直肠肛门外科,2025,31(2):125-132,8.基金项目
国家自然科学基金(81760516) （81760516）
广西自然科学基金(2019GXNSFAA185030、2023GXNSFBA026129) （2019GXNSFAA185030、2023GXNSFBA026129）
广西壮族自治区卫健委自筹经费科研课题(Z20211535、Z-A20230556) （Z20211535、Z-A20230556）
