动物医学进展2025，Vol.46Issue(6)：1-6,6.
猪肺炎支原体脂蛋白LppB介导纤毛黏附功能域的鉴定
Identification of Functional Motif in Mycoplasma hyopneumoniae LppB Mediating Ciliary Adhesion
摘要
Abstract
Mycoplasma hyopneumoniae primarily induces endemic pneumonia,resulting in substantial eco-nomic losses to the global swine industry.LppB,a virulence determinant of M.hyopneumoniae,is intricately associated with ciliary adhesion and colonization within the respiratory tract.However,the precise function-al motif remains unreported.The present study employed bioinformatics methodologies to predict the possi-ble putative functional motif,tandem repeat regions,epitope,and secondary structure of LppB.In order to maintain the integrity of the function motif,LppB is partitioned into three truncated segments,namelyΔLppB27-247,ΔLppB248-424 and ΔLppB425-604.Three truncated proteins were expressed via the prokaryotic ex-pression system,and the expression products of rΔLppB27-247,rΔLppB248-424,and rΔLppB425-604 were obtained in vitro.The adhesion ability of truncated fragments to cilia was quantified using a ciliary adhesion assay.The results demonstrated that the functional motif responsible for LppB adhesion to cilia were primarily localized within the 27-247 aa and 425-604 aa regions.This study significantly enhances our comprehension of the pathogenic mechanism of M.hyopneumoniae.关键词
猪肺炎支原体/LppB/纤毛黏附/功能域Key words
Mycoplasma hyopneumoniae/LppB/Ciliary adhesion/Functional motif分类
畜牧业引用本文复制引用
叶利颖,刘威,闫安,周丹娜,杨克礼,高婷,田永祥,宋淇淇..猪肺炎支原体脂蛋白LppB介导纤毛黏附功能域的鉴定[J].动物医学进展,2025,46(6):1-6,6.基金项目
国家自然科学基金项目(32202760) （32202760）
天津市自然科学基金项目(19JCQNJC13700) （19JCQNJC13700）
湖北省农业科技创新中心资助项目(2019-620-000-001-17) （2019-620-000-001-17）
