动物医学进展2025，Vol.46Issue(6)：7-12,6.
空置猪场的猪流行性腹泻病毒检测
Detection of Porcine Epidemic Diarrhea Virus in Vacant Swine Farm
摘要
Abstract
In order to investigate the distribution pattern of porcine epidemic diarrhea virus(PEDV)in the environment of large-scale swine farms,and to develop precise and effective biosecurity measures for swine farms,this study used the real-time fluorescent quantitative PCR method to detect 366 environmental sam-ples collected from a vacant swine farm in Xinyang cty,Henan province,China.The nucleic acid test results revealed that some environmental samples in the swine farm were positive for PEDV,with the detection rates of PEDV from high to low in different areas as follows:50％(4/8)in the sewage system,33.9％(116/342)in the swine house environment,and 25％(4/16)in the cleaning and disinfection center.Of note,the detection rates for the speed bumps in the cleaning and disinfection center,the leaking manure floors in the swine house environment,and the manure ditch in the sewage system were all 100％.This in-dicated that a amount of PEDV nucleic acid residues remained in the swine farm even after it had been thoroughly cleaned and disinfected for 6 months.The study identified blind spots in the disinfection process of the swine farm,discussed the relationship between virus residues,swine farm and swine house construc-tion planning,and various factors,and pointed out the loopholes in the existing cleaning procedures of the swine farm,providing valuable insights for establishing a comprehensive biosecurity and control system for swine farms.关键词
空置猪场/猪流行性腹泻病毒/猪场环境/清洗消毒盲区Key words
Vacant swine farm/Porcine epidemic diarrhea virus(PEDV)/Swine farm environment/Disinfec-tion blind spots分类
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李阳,张越,路浩,邢金超,李振坤,董芳婷,徐盟龙,袁晋,魏战勇..空置猪场的猪流行性腹泻病毒检测[J].动物医学进展,2025,46(6):7-12,6.基金项目
河南省科技攻关项目(242102110005,242102110078) （242102110005,242102110078）
国家自然科学基金项目(32130106) （32130106）
国家自然科学基金区域创新发展联合基金重点项目(U22A20520) （U22A20520）
