动物医学进展2025，Vol.46Issue(6)：13-18,6.
绵羊清道夫受体A启动子活性分析
Analysis of Promoter Activity of Sheep Scavenger Receptor A
摘要
Abstract
This study is to preliminarily analyze the regulatory mechanism of the promoter activity of sheep scaven-ger receptor A(SRA),laying the foundation for further exploration of the transcriptional regulation mechanism of SR A.The SRA promoter reporter plasmid was construct using homologous recombination method,validated by XhoⅠ enzyme digestion,and further sequence alignment;The constructed SRA promoter reporter plasmid was co-transfected with a marine luciferase vector into HEK293T cells,and the SRA promoter activity was detected using a dual luciferase reporter gene assay.The chemical synthesis techniques were used to synthesize eukaryotic expres-sion vectors for tumor necrosis factor alpha(TNF-α)and interleukin-6(IL-6),the SRA reporter gene vector was co-transfected with expression vectors for IRF1,TNF-α,and IL-6 in HEK293T cells,and the effects of IRF1,TNF-α and IL-6 on SRA promoter activity were analyzed.The results showed that XhoⅠ enzyme digestion of pGL3 SRA resulted in the expected target band and vector fragment,and sequencing alignment was right.The pGL3 SRA pro-moter reporter gene vector was successfully constructed;The dual luciferase reporter gene experiment confirmed that the SRA promoter has strong activity,and overexpressions of IRF1,TNF-α and IL-6 can significantly activate the promoter activity of SRA(P＜0.01,P＜0.01).This provides basic information for further exploring the regula-tory mechanisms of IRF1,TNF-α and IL-6 on SRA expression.关键词
绵羊/清道夫受体A/转录调控/启动子活性/双荧光素酶Key words
Sheep/Scavenger receptor A/Transcription regulation/Promoter activity/Dual luciferase分类
畜牧业引用本文复制引用
曹鑫艳,王钢,张秦川,范文雨,顾兰英,盛金良,贺笋,孙延鸣,张彦兵..绵羊清道夫受体A启动子活性分析[J].动物医学进展,2025,46(6):13-18,6.基金项目
国家自然科学基金项目(32360901,32060133) （32360901,32060133）
新疆生产建设兵团指导性科技计划项目(2023ZD042) （2023ZD042）
新疆维吾尔自治区科学基金项目(2022D01B197) （2022D01B197）
新疆维吾尔自治区两区项目(2023LQ03003) （2023LQ03003）
石河子大学高层次人才科研启动项目(RCZK202043) （RCZK202043）
