动物医学进展2025，Vol.46Issue(6)：18-25,8.
猫传染性腹膜炎病毒S和3c基因遗传进化分析及其S蛋白免疫原性研究
Phylogenetic Analysis of Feline Infectious Peritonitis Virus S and 3c Genes and Immunogenicity of Spike Protein
摘要
Abstract
Feline infectious peritonitis virus(FIPV),a member of the genus Coronavirus,is a pathogen that causes feline infectious peritonitis(FIP).FIPV infection poses a great threat to the health of cats.Elucida-tion of FIPV genetic variation and immunogenicity of major antigen proteins is of great significance for the prevention and control of FIP.The present study amplified S and 3c genes of four FIPV strain isolated from Shanxi province,analyzed the homology and genetic evolution characteristics of FIPV Shanxi strains.The results showed that the S gene similarity among the four FIPV Shanxi strains was 89.6％to 91.9％,and the similarity with the published type Ⅰ feline coronavirus(FCoV)was 85.9％to 92.7％.The similarity of 3c gene among FIPV Shanxi strains was 91.5％to 95.3％,and the similarity with the published type ⅠFCoV reference strains was 90.9％to 95.9％.The genetic and evolutionary analysis of S and 3c genes showed that all the four FIPV Shanxi strains belonged to the type Ⅰ FCoV.FIPV S protein was expressed,purified and used to immunize Balb/c mice.Indirect ELISA was used to detect the anti-FIPV-S protein anti-body in mice.The results showed that FIPV S protein had good immunogenicity,which provided an impor-tant scientific basis for the diagnosis and prevention of FIPV infection.关键词
猫传染性腹膜炎病毒/遗传进化分析/刺突蛋白/3c蛋白/免疫原性Key words
Feline infectious peritonitis virus/Genetic and evolutionary analysis/Spike protein/3c protein/Immunogenicity分类
畜牧业引用本文复制引用
张婷,刘童通,李春雨,姚孟,李炜,杨勃,孙子龙,牛胜,张鼎..猫传染性腹膜炎病毒S和3c基因遗传进化分析及其S蛋白免疫原性研究[J].动物医学进展,2025,46(6):18-25,8.基金项目
山西省基础研究计划项目(202303021211105) （202303021211105）
山西省科技创新人才团队专项资助项目(202304051001041,202204051001022) （202304051001041,202204051001022）
