动物医学进展2025，Vol.46Issue(6)：31-35,5.
oar-miR-103和oar-miR-107通过靶向IL-1β基因区域调节其表达
oar-miR-103 and oar-miR-107 Regulate Expressions of IL-1β Gene by Targeting Their Regions
摘要
Abstract
To investigate whether sheep microRNAs(miRNAs)target the IL-1β gene,and to initially deter-mine the specific region of the miRNA that targets its gene,and to verify the regulatory effect of miRNAs on IL-1β expression,taking sheep miRNAs as the research object,the miRanda software was used to ana-lyze the specific regions and binding ability of miRNAs targeting the IL-1β gene.The sheep IL-1β gene was connected to a luciferase reporter gene vector,and the targeting effect of oar-miR-103 and oar-miR-107 on the IL-1β gene region was verified through a luciferase reporter gene experiment.Primary sheep alveolar macrophages were isolated and overexpressed with oar-miR-103 and oar-miR-107 to analyze the IL-1β tran-script expression level.The results showed that oar-miR-103 and oar-miR-107 targeted two and three re-gions of the IL-1β gene,respectively.A luciferase reporter vector pGL3-promoter-IL-1β was successfully constructed,and the dual luciferase assay was used to detect the targeting effect of oar-miR-103 and oar-miR-107 on the IL-1β gene region.It was found that compared with the miR-NC group,both oar-miR-103 and oar-miR-107 groups significantly decreased the activity of pGL3-promoter-IL-1β.Cell experiments dem-onstrated that oar-miR-107 significantly inhibited the transcription and expression of IL-1 β mRNA.The re-sults demonstrated that oar-miR-103 and oar-miR-107 have the ability to target and inhibit the expression of IL-1β gene,providing basic information for further exploring the regulation of miRNAs on IL-1β expres-sion.关键词
白介素1β/microRNAs/miRanda/荧光素酶报告基因Key words
IL-1β/MicroRNAs/miRanda/Luciferase reporter gene分类
畜牧业引用本文复制引用
高之煜,孙延鸣,顾兰英,范文雨,曹鑫艳,陈创夫,肖非,易继海,盛金良,张彦兵..oar-miR-103和oar-miR-107通过靶向IL-1β基因区域调节其表达[J].动物医学进展,2025,46(6):31-35,5.基金项目
国家自然科学基金项目(32360901,32060133) （32360901,32060133）
兵团指导性科技计划项目(2023ZD042)) （2023ZD042）
新疆维吾尔自治区青年科学基金项目(2022D01B197) （2022D01B197）
石河子大学高层次人才科研启动项目(RCZK202043) （RCZK202043）
