慢性鼻窦炎伴鼻息肉复发患者血清MMP-2和IL-25表达与组织中肥大细胞浸润的相关性研究OA

OBJECTIVE To explore the correlation between serum matrix metalloproteinase-2(MMP-2),interleukin-25(IL-25)expression and mast cell infiltration in patients with recurrent chronic sinusitis with nasal polyps(CRSwNP).METHODS Sixty patients diagnosed with recurrent CRSwNP in our hospital from October 2022 to March 2023 were selected as the CRSwNP group,and 60 patients diagnosed with simple Nasal septal deviation(NSD)in our hospital during the same period were selected as the NSD group.Compare the clinical data,mast cell count,and serum MMP-2 and IL-25 expression levels between two groups of patients.Use local weighted regression(Lowess)to analyze the correlation between MMP-2,IL-25 levels and mast cell count.Apply multiple logistic regression analysis to investigate the independent correlation between MMP-2,IL-25 levels and the risk of CRSwNP recurrence.Establish a restricted cubic spline(RCS)model to analyze the dose-response relationship between serum MMP-2,IL-25 levels and the risk of CRSwNP recurrence.Analyze the interaction and type of interaction between MMP-2,IL-25,and mast cell count on the risk of CRSwNP recurrence using an interaction calculation table.RESULTS The Lund Kennedy endoscopic score,Lund Mackay CT score,total VAS score,peripheral eosinophils,peripheral basophils,inhaled allergens,total IgE levels,smoking,alcohol consumption,and incidence of asthma,mast cell count,serum MMP-2 and IL-25 levels in the CRSwNP group were significantly higher than those in the NSD group,and the differences were statistically significant(P<0.05).Lowess analysis showed that there was a nonlinear relationship between MMP-2,IL-25 and mast cell count.MMP-2 and IL-25 were independently correlated with the recurrence of crswnp.RCS model analysis showed that MMP-2 and IL-25 levels had a nonlinear dose-response relationship with the recurrence risk of crswnp(Pfor non linear=0.002,0.008).The recurrence rates of crswnp corresponding to MMP-2,IL-25 and mast cell count in high-level group were 5.26%,12.54%and 19.08%,respectively,which were significantly higher than those corresponding to MMP-2,IL-25 and mast cell count in low-level group(55.91%,70.83%and 68.05%),and the difference was statistically significant(P<0.05).The results of interaction analysis showed that MMP-2,IL-25 and mast cell count had interaction in the additive model and the multiplicative model,showing a synergistic effect.CONCLUSION The levels of serum MMP-2 and IL-25 in CRSwNP recurrent patients are positively correlated with the degree of mast cell infiltration in the tissue.The higher the levels of MMP-2,IL-25 and Mast cell count,the higher the recurrence rate of CRSwNP.There was an independent correlation between MMP-2,IL-25 and recurrence of CRSwNP,which was a risk factor for recurrence of CRSwNP.