动物医学进展2025，Vol.46Issue(6)：36-41,6.
鼠伤寒沙门氏菌孔蛋白突变株的致病性研究
Study on the Pathogenicity of a Mutant Strain of Salmonella typhimurium Pore Protein
摘要
Abstract
Pore proteins have important roles in immunogenicity and virulence determinants.In order to in-vestigate whether mutations in the pore protein ompD,ompS,and ompW genes in Salmonella have an effect on the pathogenicity of Salmonella,this study was conducted on the basis of constructing Salmonel-la typhimurium pore protein mutant strains STM LT2 ΔompD,STM LT2 ΔompS,and STM LT2ΔompW,and its mouse pathogenicity,in vivo genetic stability,LD50,and hepatic and splenic loads were were determined.The results showed that both the standard strain and the mutant strains were pathogenic to mice,and the sizes of liver and spleen increased significantly;the mutant strains were still genetically stable in mice for 20 consecutive generations;the LD50 toxicity assay revealed that the virulence of the mu-tant strains showed a trend of weakening,with the most obvious weakening of the virulence of the mutant strain STM LT2 ΔompW;and the liver and spleen load assay revealed that,compared with the standard strain,the mutant strains were distributed in the liver and spleen of mice,with a greater distribution in the liver and spleen.The bacterial loads in the liver and spleen of the mutant strains were found to be signifi-cantly reduced compared with the standard strain.This experiment lays the foundation for the development of Salmonella typhimurium attenuated vaccine,and also provides a reference for exploring the molecular mechanism of Salmonella typhimurium pathogenicity.关键词
鼠伤寒沙门氏菌/孔蛋白/致病性Key words
Salmonella typhimurium/Pore protein/Pathogenicity分类
畜牧业引用本文复制引用
武绍碧,令狐远凤,潘永,杨婉,陈世雄,叶景芬,杨琦..鼠伤寒沙门氏菌孔蛋白突变株的致病性研究[J].动物医学进展,2025,46(6):36-41,6.基金项目
国家自然科学基金项目(32260884) （32260884）
