动物医学进展2025，Vol.46Issue(6)：42-46,5.
鸽源金黄色葡萄球菌外毒素基因与细菌分子分型相关性研究
Correlation Between Exotoxin Genes of Pigeon Origin Staphylococcus aureus and Bacterial Molecular Typing
摘要
Abstract
To study the toxin genes,agr and spa genotypes of Staphylococcu aureus derived from pigeons and to analyze the correlation between its typing and toxin genes,the agr typing of the isolates was detected by multiplex PCR,and spa sequencing was performed.The results showed that:(1)The detection rate of hla and hlb in toxin genes were high;The detection rates of sec,seg and seh were 16.00％.The lowest detectable rates of sea,seb,sed,see and tst were 2.00％;MRSA strains carry four or more toxin genes.(2)Genotyping results showed that among the agr subtypes of S.aureus,agr Ⅰ was the dominant strain of S.aureus derived from pigeons,accounting for 70.00％of the isolation rate;The detection rates of agr Ⅱ and agr Ⅲ were 12.00％and 18.00％,respectively.agr Ⅳ was not detected.Nine subtypes were detected by spa gene.t011 type S.aureus was the main epidemic strain of pigeon origin,accounting for 68.00％,followed by t127 and t502,which were both 10.00％.agr Ⅱ and agr Ⅲ S.aureus have more potential to carry multiple toxin genes,while the carrying capacity of agr Ⅰ toxin gene is weak.S.aureus of type t011 all carry the hla gene,and the MRSA strains of types t502 and t1710 all carry seven toxin genes,indicating that condition of the toxin genes carried by the isotypes is consistent.t011 all carried agr Ⅰ,t127 and t502 all carried agr Ⅲ.It indicated that there was a correlation between spa classification and agr classification.In this study,the potential ability to carry toxin genes was analyzed by S.aureus typing test,which would provide a scientific basis for the monitoring of pigeon-derived S.aureus infections and the research of molecular epidemiology.关键词
金黄色葡萄球菌/毒素基因/辅助基因调节子/蛋白AKey words
Staphylococcus aureus/Toxin gene/agr/spa分类
农业科技引用本文复制引用
赵世芳,王金泉,陈伟丽,阮志栋,彭斌..鸽源金黄色葡萄球菌外毒素基因与细菌分子分型相关性研究[J].动物医学进展,2025,46(6):42-46,5.基金项目
中央引导地方科技发展专项(ZYYD2022C13) （ZYYD2022C13）
新疆维吾尔自治区"三农"骨干人才培养项目(2022SNGGGCC015) （2022SNGGGCC015）
