动物医学进展2025，Vol.46Issue(6)：47-56,10.
26种中药抗PEDV感染IPEC-J2细胞的筛选效果分析
Analysis of Screening Effect of 26 Kinds of Traditional Chinese Medicine against PEDV-infected IPEC-J2 Cells
摘要
Abstract
As the porcine epidemic diarrhea virus(PEDV)features high variability and causes a high mor-tality rate among piglets,the protection rate of vaccine immunity against variant strains is low,and the treatment cost of Western medicine is high.Currently,seeking Chinese herbal medicines against PEDV has become a crucial research point nowadays.Through the TCID50 method to observe the viral titer,it was con-cluded that as the infection time of PEDV on intestinal porcine epithelial cell line-J2(IPEC-J2)increased,the viral titer gradually rose.HE observation revealed that after PEDV infected IPEC-J2 cells,shrinkage oc-curred,the intercellular space enlarged,and the phenomena of drawing silk and rounding up were observed.The CCK8 method was employed to detect the maximum safe concentration of the Chinese medicinal liquid in IPEC-J2 cells.Through calculating the virus inhibition rate,it was found that the water decoction of An-drographis paniculata(CXL)was the best in blocking the effect of PEDV,with a virus inhibition rate as high as 57.8％.The water decoction of roasted Atractylodes Rhizome(CBZ)had the best effect on directly inactivating the virus,with a virus inhibition rate as high as 51.9％.The water decoction of Codonopsis pi-losula(DS)had the best inhibitory effect,with a virus inhibition rate as high as 60.7％.DS groups with dif-ferent doses(DS 1.25,2.5 and 5 mg/mL)were designed to detect the mRNA expression levels of PEDV N,IL-1β,IL-2,IL-6 and TNF-α in PEDV-infected IPEC-J2 cells by RT-PCR.It was found that DS inhibited PEDV N gene in a concentration-dependent manner,and DS significantly decreased PEDV N mRNA expression level in PEDV-infected IPEC-J2 cells(P＜0.01).Compared with low and medium doses of DS(DS 1.25,2.5 mg/mL),high dose of DS could significantly reduce the mRNA expression levels of IL-1β,IL-2,IL-6 and TNF-α in PEDV-infected IPEC-J2 cells.The contents of IL-1β,IL 2,IL-6 and TNF-α proteins were detected by ELISA.Compared with low and medium doses of DS,it was concluded that high-dose DS could better reduce the contents of IL-1β,IL-2,IL-6 and TNF-α proteins after PEDV infection of IPEC-J2 cells.The results showed that DS had the best inhibitory effect on virus,among which high dose DS had a significant inhibitory effect on PEDV replication and could alleviate the inflammatory response of PEDV to IPEC-J2 cells.关键词
中药/猪流行性腹泻病毒/猪肠上皮细胞/病毒抑制率/抗病毒作用/炎症Key words
Traditional Chinese medicine/Porcine epidemic diarrhea virus/IPEC-J2 cell/Virus inhibition rate/Antiviral effect/Inflammation分类
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任涛,李俊贤,郭杨,文安林,张依裕,欧德渊..26种中药抗PEDV感染IPEC-J2细胞的筛选效果分析[J].动物医学进展,2025,46(6):47-56,10.基金项目
贵州省生猪产业高质量发展项目(黔财农[2022]183号,黔财农[2023]78号) （黔财农[2022]183号,黔财农[2023]78号）
