动物医学进展2025，Vol.46Issue(6)：57-64,8.
抗奶山羊源葡萄球菌藏药筛选及对小鼠乳房炎的治疗作用研究
Screening of Tibetan Drugs against Dairy Goat Staphylococcus and Study of Therapeutic Effect on Mouse Mastitis
摘要
Abstract
Dairy goat mastitis is one of the major diseases affecting dairy goat farming.Currently,antibiotics are widely used to treat dairy goat mastitis,but long-term use of antibiotics has led to bacterial resistance and veterinary drug residues and other public health problems.Therefore,the search for alternative antibi-otic drugs to treat mastitis is of great significance.In this study,9 kinds of Tibetan medicines with antibac-terial,antiviral and immunomodulatory effects were selected and their effective components were extracted by water method.Then,the bacteriostatic effect in vitro was studied by co-culture of water extract and bac-teria.At the same time,SPF Kunming mice were used to verify the safety of Tibetan medicine water ex-tracts,Staphylococcus aureus was used to construct mouse mastitis animal model,and Tibetan medicine proved to have in vitro antibacterial effect was used for gavage treatment,and the pathological changes of mice were observed.In addition,the expression levels of TNF-α,IL-6,IL-8 and IL-1β in mammary tissue of mice in different groups were detected by qPCR.The results showed that the positive control of TNF-α,IL-6,IL-8 and IL-1β had significant differences compared with the negative control(P＜0.01).When the infu-sion time reached 14 days,the expressions of inflammatory factors decreased to different degrees,and the decrease of IL-1β was the most obvious,but there was no significant difference compared with the negative control group(P＞0.05).The results showed that in the Tibetan medicines selected in this experiment,the Tibetan medicine Dracocephalum tanguticum and Tribulus terrestris had obvious inhibitory effect on the four species of Staphylococcus when the original drug concentration was 1 g/mL.In addition,the safety test of mice showed that when the original drug concentration was 1 g/mL and the dosage was below 400μL,the mice in both groups did not show significant abnormalities.Meanwhile,in the treatment of Tibetan medicine,only Tibetan medicines Gan Qingqing Lan could effectively relieve the inflammation of the mam-mary tissue of mice.Therefore,according to the above test results,this study successfully screened a Tibet-an medicine with both in vivo and in vitro anti-staphylococcal effects,which provided a certain research ba-sis for the comprehensive development of Tibetan medicines for the treatment of dairy goat mastitis.关键词
奶山羊/乳房炎/藏药/动物模型Key words
Dairy goat/Mastitis/Tibetan medicine/Animal model分类
畜牧业引用本文复制引用
李启瑞,豆薇,顾庆云,李生庆,郭抗抗..抗奶山羊源葡萄球菌藏药筛选及对小鼠乳房炎的治疗作用研究[J].动物医学进展,2025,46(6):57-64,8.基金项目
陕西省农业科技创新转化项目(NYKJ-2021-YL(XN)16) （NYKJ-2021-YL(XN）
西藏自治区科技计划项目(XZ202101YD001C) （XZ202101YD001C）
青海省科技计划项目(2022-NK-118) （2022-NK-118）
