福建农业学报2025，Vol.40Issue(2)：113-122,10.DOI:10.19303/j.issn.1008-0384.2025.02.001
番茄APETALA2乙烯响应因子重鉴定及抗逆相关基因筛选
Re-identification of AP2/ERF Transcription Factors in Search for Disease Resistance-related Genes in Tomato Plant
摘要
Abstract
[Objective]Genomics and stress response of tomato AP2/ERF transcription factors(TFs)were studied for breeding applications.[Method]Based on the recently available genetic information related to bacterial wilt resistance,tomato AP2/ERF TFs were reexamined for confirmation.Using the transcriptome sequence of tomato inbred lines infected by Ralstonia solanacearum(Rs),the re-identified TFs were analyzed to search for genes related to disease-resistance.[Result]One-hundred-seventy-three AP2/ERF TFs were identified from the data bank.They were mainly in the clusters at the ends of chromosomes,especially Chromosomes 3 and 8,with 50.3%of them collinearity.Phylogenetically,the AP2 subfamily had 22 genes(i.e.,12.7%of total)in two main categories;the ERF subfamily,93 genes(i.e.,53.8%of total)in 6 categories;the DREB subfamily,49 genes(i.e.,28.3%of total)in two categories;and the RAV subfamily,3 genes.The Ka/Ks of the 71 pairs of tomato AP2/ERF homologs were all lower than 1.Of the AP2/ERF TFs,26.8%were expressed largely in the roots and more highly under certain biotic stresses in the ERF_4-6 and DREB_2 categories,especially Solyc03g005500.1(ERF_6)and Solyc08g078170.1(ERF_5).Ten AP2/ERF TFs,belonging separately to AP2 and ERF,differentially expressed in the Rs-resistant and Rs-susceptible tomato lines.Among them,Solyc06g068570.4(AP2)was positively regulated,and the remainders negatively regulated.The other 50.9%of the AP2/ERF TFs became 193 interacting pairs,of which the one between Solyc01g096860.3(AP2_2)and Solyc08g078190.2(ERF_5)was most reliable.The positive and negative interactions between Solyc06g068570.4(AP2)and Solyc03g093560.1(ERF_5)might also involve in the response of a tomato plant to the bacterial wilt.[Conclusion]The AP2/ERF TFs associated with the response of a tomato plant to bacterial wilt were confirmed using the newly available information on the genome and transcriptome.By studying the differential genes in the Rs-resistant and Rs-susceptible inbred tomato lines,specific genes were identified for further studies and breeding applications.关键词
番茄/AP2/ERF转录因子/基因组特征/生物胁迫/转录表达Key words
tomato/AP2/ERF transcription factor/genomic characteristics/biotic stress/transcriptional expression分类
园艺学与植物营养学引用本文复制引用
史建磊,黄少勇,陈先知,宰文珊,熊自立..番茄APETALA2乙烯响应因子重鉴定及抗逆相关基因筛选[J].福建农业学报,2025,40(2):113-122,10.基金项目
浙江省农业新品种选育重大科技专项(2021C02065) （2021C02065）
温州市农业新品种选育协作组项目(ZX2024002-2) （ZX2024002-2）
国家大宗蔬菜产业技术体系建设专项(CARS-23-G45) （CARS-23-G45）
