福建农业学报2025，Vol.40Issue(2)：143-150,8.DOI:10.19303/j.issn.1008-0384.2025.02.004
鹅水禽圆环病毒感染调查及自然共感染病例病毒基因组分子特征
Epidemiology of Waterfowl Circoviral diseases and Characteristics of Genomes in Natural Cross-species Infection on Goose
摘要
Abstract
[Objective]The prevalence of waterfowl circoviruses(WFCVs)including goose circovirus(GoCV)and duck circovirus(DuCV)at geese farms was surveyed,and molecular characteristics of the GoCV and DuCV genomes in the first case of natural co-infection on geese studied.[Method]From the flocks at geese farms in 6 provinces,153 tissue specimens were collected during 2023-2024.GoCV and DuCV were detected by using a tri-primer PCR method.Genome sequences of the viruses in samples of the first natural co-infection case were determined.[Result]Fifty-three WFCVs were identified from the collected specimens at a positive rate of 34.6%.Of which,47 were positive for GoCV,5 for DuCV,and one from Anhui Province(named as AH2367)with both GoCV and DuCV.The whole genome sequence of Go-AH2367 was 1821 nt in length with a homology of 82.9%-99.0%with GoCV and 67.0%-70.9%with DuCV,while that of Du-AH2367 was 1992 nt long with a homology of 84.4%-99.7%with DuCV and 66.1%-68.8%with GoCV.The two major functional proteins of the Go-AH2367 and Du-AH2367(Rep and Cap)were consisted of highly conserved critical motifs with a few sporadic site variations but free of gene recombination,site deletion or insertion on the amino acid sequences.The phylogenetic tree of Go-AH2367 was clustered into an evolutionary branch of the Genotype 1 GoCV isolates,while that of Du-AH2367 of the Genotype I DuCV isolates in the same evolutionary branch.[Conclusion]The WFCV infections in geese occurred in China were severe.They were mostly the GoCV type with only one confirmed cross-species case that could cause a diverse epidemic.The genomes of Go-AH2367 and Du-AH2367 found in the co-infected goose were genetically stable with no apparent variations by deletion or recombination.关键词
鹅圆环病毒/鸭圆环病毒/跨种感染/共感染/分子特征Key words
goose circovirus/duck circovirus/cross-species infection/co-infection/molecular characteristics分类
畜牧业引用本文复制引用
林永强,陈红梅,江南松,王威威,林建生,郑小兰,黄瑜,傅光华,李卫,梁齐章,傅秋玲,焦文龙,曹申,程龙飞,万春和,刘荣昌..鹅水禽圆环病毒感染调查及自然共感染病例病毒基因组分子特征[J].福建农业学报,2025,40(2):143-150,8.基金项目
国家自然科学基金项目(32372995) （32372995）
国家水禽产业技术体系建设专项(CARS-42) （CARS-42）
中央引导地方科技发展专项(2023L3023) （2023L3023）
福建省科技计划公益类专项(2023R1024005) （2023R1024005）
福建省科技计划项目(2020J06029) （2020J06029）
福建省农业科学院科技创新团队建设项目(CXTD2021005-1) （CXTD2021005-1）
福建省农业科学院农业科技专项(GJYS202411) （GJYS202411）
