福建农业学报2025，Vol.40Issue(2)：189-197,9.DOI:10.19303/j.issn.1008-0384.2025.02.009
百香果根际土壤中拮抗细菌的筛选及其促生与抗病特性
Species,Growth-promoting,and Disease-resistance of Antagonistic Microbes in Passion Fruit Rhizosphere Soil
摘要
Abstract
[Objective]Microorganisms antagonistic to Fusarium solani were isolated from the rhizosphere soil of healthy passion fruit plants and studied for potential application as a biocontrol agent.[Method]Rhizosphere oil at the field of healthy passion fruit plants was sampled to isolate microbes antagonistic to F.solani using the confrontational culture method.Morphological observation and molecular identification were performed to determine the species and biological characteristics of the isolates.Potential as a biocontrol agent of the identified strains was evaluated on the extracellular enzyme detection plates,while possible growth promoting effect examined on tomato seeds.[Result]Eight microbial strains antagonistic to F.solani were isolated from 7 soil specimens.Among them,NN-Q03-10,SL-Q02-05,and SL-Q02-06 exhibited the greatest inhibition rates of 57.81%,57.34%,and 57.34%,respectively.Four of them belonged to Bacillus subtilis,and 4 to B.velezensis.All could produce proteases,cellulases,β-1,3-glucanases,and xylanases with genomes consisting of antibiotic bacA,FenB,and yanJ.In addition to those genes,SL-Q02-05,NN-Q03-01,NN-Q03-09,and NN-Q03-10 also contained MycB and ifuA.Validation of growth promoting effects using tomato seeds,and the results showed that six strains(SL-Q02-04、SL-Q02-05、SL-Q02-06、SL-Q03-01、NN-Q03-01、NN-Q03-10)could promote embryonic root growth,among them,SL-Q02-04 had the most significant promoting effect with a embryonic root length of 7.32 cm.[Conclusion]The 8 Bacillus strains antagonistic to F.solani were identified.Their ability in producing protease,cellulase,β-1,3-glucanase,and xylanase and some of them having genomes containing mycB,ifuA,FenB,bacA,and yndJ related to antibiotic property strongly suggested a potential in serving as a biocontrol agent for F.solani infection on plants.关键词
百香果/茎基腐病/腐皮镰刀菌/筛选鉴定/生物防治Key words
Passion fruit/base stem rot/Fusarium solani/isolation and identification/biocontrol agent分类
植物保护学引用本文复制引用
覃俏惠,黄浩华,金铭,蒙姣荣,李界秋..百香果根际土壤中拮抗细菌的筛选及其促生与抗病特性[J].福建农业学报,2025,40(2):189-197,9.基金项目
广西重点研发计划项目(桂科AB21220001) （桂科AB21220001）
广西农垦科技项目(KHKY202404) （KHKY202404）
