柳枝稷(Panicum virgatum)莽草酸脱氢酶基因电子克隆及分析OA北大核心

Shikimate dehydrogenase(AroE)is a key enzyme in the biosynthetic pathway of ar-omatic amino acids,catalyzing the conversion of 3-dehydroshikimate to shikimate.Analyzing the AroE structure helps elucidate its biocatalytic mechanism.Using the shikimate dehydrogenase gene from Zea mays as a probe,high-homology expressed sequence tag(EST)sequences were screened and assembled to obtain the complete cDNA sequence.The amino acid sequence was predicted through ORF analysis and further examined for its physicochemical properties,protein-protein inter-actions,transmembrane structure,phosphorylation sites,and signal peptides.The AroE of Pani-cum virgatum is encoded by 355 amino acids and is a hydrophilic,cytoplasmic protein without transmembrane domains or signal peptides.The phylogenetic tree indicates that the AroE of Panicum virgatum clusters with that of Panicum hallii.