福建师范大学学报（自然科学版）2025，Vol.41Issue(3)：97-102,110,7.DOI:10.12046/j.issn.1000-5277.2024120012
柳枝稷(Panicum virgatum)莽草酸脱氢酶基因电子克隆及分析
In Silico Cloning and Analysis of the Shikimate Dehydrogenase Gene from Panicum virgatum
摘要
Abstract
Shikimate dehydrogenase(AroE)is a key enzyme in the biosynthetic pathway of ar-omatic amino acids,catalyzing the conversion of 3-dehydroshikimate to shikimate.Analyzing the AroE structure helps elucidate its biocatalytic mechanism.Using the shikimate dehydrogenase gene from Zea mays as a probe,high-homology expressed sequence tag(EST)sequences were screened and assembled to obtain the complete cDNA sequence.The amino acid sequence was predicted through ORF analysis and further examined for its physicochemical properties,protein-protein inter-actions,transmembrane structure,phosphorylation sites,and signal peptides.The AroE of Pani-cum virgatum is encoded by 355 amino acids and is a hydrophilic,cytoplasmic protein without transmembrane domains or signal peptides.The phylogenetic tree indicates that the AroE of Panicum virgatum clusters with that of Panicum hallii.关键词
柳枝稷/莽草酸途径/莽草酸脱氢酶/电子克隆/同源性分析/代谢机制Key words
Panicum virgatum/shikimate pathway/shikimate dehydrogenase/in silico clo-ning/homology analysis/metabolic mechanism分类
农业科技引用本文复制引用
夏煌慧,黄建忠..柳枝稷(Panicum virgatum)莽草酸脱氢酶基因电子克隆及分析[J].福建师范大学学报（自然科学版）,2025,41(3):97-102,110,7.基金项目
国家重点研发计划项目(2022YFD1802104) （2022YFD1802104）
