福建中医药2025，Vol.56Issue(4)：24-26,3.DOI:10.13260/j.cnki.jfjtcm.2025.04005
电针后海穴调控CREB/BDNF信号通路对缺血缺氧脑损伤大鼠海马CA1区突触可塑性的影响
CREB/BDNF Signaling Pathway on Synaptic Plasticity of the Hippocampal CA1 Region in Rats With Hypoxic-Ischemic Brain Injury
摘要
Abstract
Objective:To observe the effect of electroacupuncture at Houhai acupoint on synaptic plasticity in the hippocampal CA1 region of rats with hypoxic-ischemic brain injury by regulating the CREB/BDNF signaling pathway.Methods:A total of eight female SD rats and four male SD rats were mated for two weeks.Forty seven-day-old SD pups were randomly divided into blank group(n=8),sham surgery group(n=8),and modeling group(n=24).The blank group received no modeling intervention.In the sham surgery group,the left common carotid artery of the pups was separated,and the wound was sutured without further hypoxic treat-ment.The model of cerebral palsy was established by Rice-Vannucci method,that is,the left common carotid artery of pups in the modeling group was separated ligated and cut,followed by hypoxic treatment.Successful modeling was determined by Longa score of≥2 after 24 hours.The modeling group was then randomly divided into model group,non-acupoint group,and Houhai acupoint group(n=8).Interventions were performed the next day.The Houhai acupoint group received electroacupuncture at the Houhai acu-point,while the non-acupoint group received electroacupuncture at the intersection point approximately 15 mm above the iliac crest and 20 mm lateral to the posterior midline.The blank,sham surgery,and model groups received no electroacupuncture intervention.After 10 days of treatment,Morris water maze tests were conducted on all five groups of pups to measure escape latency and platform crossings numbers.Immunohistochemistry was used to detect the protein expression levels of cAMP response element-binding protein(CREB),phosphorylated cAMP response element-binding protein(p-CREB),and brain-derived neurotrophic factor(BDNF)in the hippocampal CA1 region of the five groups.Results:There were no statistically significant differences in escape latency,platform crossing numbers,or protein expression levels of CREB,p-CREB,and BDNF between the sham surgery group and the blank group(P>0.05).Compared with the sham surgery group,the model group had prolonged escape latency(P<0.05),reduced platform cross-ings(P<0.05),and decreased protein expression levels of CREB,p-CREB,and BDNF(P<0.05).Compared with the model group,the Houhai acupoint group had shortened escape latency(P<0.05),increased platform crossing numbers(P<0.05),and elevated protein ex-pression levels of CREB,p-CREB,and BDNF(P<0.05).Compared with the non-acupoint group,the Houhai acupoint group had re-duced escape latency,increased platform crossing numbers(P<0.05),and higher protein expression levels of CREB,p-CREB,and BDNF(P<0.05).Conclusion:Electroacupuncture at the Houhai acupoint can improve learning and memory abilities in rats with hy-poxic-ischemic brain injury,and its mechanism is related to the upregulation of the CREB/BDNF signaling pathway.关键词
缺血缺氧脑损伤/CREB/BDNF通路/海马CA1区/突触可塑性/后海穴Key words
hypoxic-ischemic brain injury/CREB/BDNF signaling pathway/hippocampal CA1 region/synaptic plasticity/Hou-hai acupoint引用本文复制引用
林雨芳,戴永辉..电针后海穴调控CREB/BDNF信号通路对缺血缺氧脑损伤大鼠海马CA1区突触可塑性的影响[J].福建中医药,2025,56(4):24-26,3.基金项目
福建省自然科学基金项目(2022J011309) （2022J011309）
