分析测试学报2025，Vol.44Issue(5)：822-835,14.DOI:10.12452/j.fxcsxb.241030499
基于UPLC-Q-TOF-MS/MS技术的延龄益寿方化学成分系统筛选与鉴定
Systematic Screening and Identification of Chemical Constituents in Yan Ling Yi Shou Formula Based on UPLC-Q-TOF-MS/MS Technology
摘要
Abstract
Ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrome-try(UPLC-Q-TOF-MS/MS)technology was used to systematically and comprehensively characterize the chemical components of Yan Ling Yi Shou Formula(YLYSF).A Waters ACQUITY UPLC® HSS T3 column(2.1 mm×100 mm,1.8 μm)was used for the separation,with an injection volume of 5 μL and mobile phases of 0.1%formic acid solution and 0.1%formic acid acetonitrile solution in a gra-dient elution at a flow rate of 250 μL/min.Both positive and negative ion monitoring modes were im-plemented for the electrospray ionization source(ESI).The identification of compounds was conduct-ed through the MS and MS/MS data,reference standards,database,and literature reports.113 chemicals in all were examined and determined from YLYSF,including 32 terpenoids,24 flavo-noids,16 phenylpropanoids,12 organic acids,4 benzoquinones and 25 other compounds.This study laid the foundation for the chemical substances of YLYSF.The results of this study can serve as a basis for further research on the development of standards for evaluating quality,the selection of active ingre-dients,and the clarification of the formula's mechanism of action.关键词
延龄益寿方/UPLC-Q-TOF-MS/MS技术/系统筛选/化学成分/裂解规律Key words
Yan Ling Yi Shou Formula/UPLC-Q-TOF-MS/MS/systematic screening/chemical constituents/fragmentation pattern分类
化学引用本文复制引用
拱佳慧,向杰,邹建东,周青,李长印..基于UPLC-Q-TOF-MS/MS技术的延龄益寿方化学成分系统筛选与鉴定[J].分析测试学报,2025,44(5):822-835,14.基金项目
江苏省中医院高峰人才第三层次项目(y2021rc31) （y2021rc31）
江苏高校优势学科建设工程资助项目(PAPD-2022) （PAPD-2022）
江苏省干部保健科研项目(BJ23008) （BJ23008）
