分析测试学报2025，Vol.44Issue(5)：868-875,8.DOI:10.12452/j.fxcsxb.240816310
QuEChERS/超高效液相色谱-串联质谱法测定水产品中25种麻醉剂
Determination of 25 Anesthetics in Aquatic Products Using QuEChERS Coupling with Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
This study analyzed 25 anesthetics in aquatic products using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)in combination with QuEChERs pretreat-ment technology.Samples were extracted with acetonitrile after soaked in water and cleaned up using a sorbent mixture of octadecyl bonded silica gel(C18),N-propylethylenediamine(PSA)and carbon black(Carb).The extracts were chromatographically separated using a Waters HSS T3 column,fol-lowed by MS detection in multiple reaction monitoring(MRM)mode.Eugenol compounds were deter-mined using acetonitrile and water as mobile phases in positive and negative ESI modes,while caine anesthetics,quinalidine and diazepam were determined with a mobile phase of acetonitrile-0.1%for-mic acid water in positive ESI mode.Under optimized conditions,good linearity was observed for the 25 anesthetics in the range of 1-50 ng/mL,with specific compounds like 3-aminobenzoic acid,4-aminobenzoic acid,and 6 eugenol compounds demonstrated linearity within the ranges of 10-500 ng/mL.The limits of quantitation(S/N=10)were 1-10 μg/kg.Average recoveries at three spiked levels ranged from 65.2%to 112%,with relative standard deviations(RSDs)of 2.1%-11%(inter-batch,n=6)and 2.3%-11%(intra-batch,n=3).This method is simple,sensitive and specific,making it suitable for the rapid qualitative and quantitative analysis of anesthetics in aquatic products.关键词
QuEChERS/超高效液相色谱-串联质谱法/水产品/麻醉剂Key words
QuEChERS/ultra-performance liquid chromatography-tandem mass spectrometry/aquatic products/anesthetics分类
化学引用本文复制引用
唐韵熙,白亚敏..QuEChERS/超高效液相色谱-串联质谱法测定水产品中25种麻醉剂[J].分析测试学报,2025,44(5):868-875,8.基金项目
重庆市市场监督管理局科技项目(CQSJKJDW2023033) （CQSJKJDW2023033）
