分析化学2025，Vol.53Issue(4)：516-524,9.DOI:10.19756/j.issn.0253-3820.231402
氮掺杂碳载钌金属原子纳米酶用于黄嘌呤氧化酶活性分析及其抑制剂的比色检测
Nitrogen-doped Carbon-supported Ruthenium Metal Atom Nanozyme for Colorimetric Determination of Xanthine Oxidase Inhibitors
摘要
Abstract
A colorimetric detection method for xanthine oxidase activity and its inhibitors(XOI)based on nitrogen-doped carbon-supported ruthenium metal atom(Ru/NC)nanozyme was developed.Through a host-guest strategy,Ru/NC nanozyme with excellent peroxidase-like activity was prepared using zeolitic imidazolate framework-8(ZIF-8)as the host molecule and ruthenium acetylacetonate(Ru(acac)3)as the guest molecule.Based on its peroxidase-like catalytic activity,the visual method for detection of hydrogen peroxide(H 2O 2)and analysis of xanthine oxidase(XO)activity were developed.Under optimized experimental conditions,the linear detection range for H 2O 2 was 10-500 μmol/L,with a detection limit of 4.8 μmol/L(3σ);for XO activity analysis,the linear range was 1-7 mU/mL,with a detection limit of 0.69 mU/mL(3σ).Furthermore,an effective method for screening XOI was developed,which had the advantages of low cost,simplicity,and visualization.关键词
纳米酶/比色分析/过氧化氢/黄嘌呤氧化酶/黄嘌呤氧化酶抑制剂Key words
Nanozyme/Colorimetric analysis/Hydrogen peroxide/Xanthine oxidase/Xanthine oxidase inhibitor引用本文复制引用
刘全艺,房琦,魏辉,杜衍..氮掺杂碳载钌金属原子纳米酶用于黄嘌呤氧化酶活性分析及其抑制剂的比色检测[J].分析化学,2025,53(4):516-524,9.基金项目
国家自然科学基金项目(Nos.21874129,22322410)、吉林省科技厅重点研发项目(No.20210204126YY)、长春市科技局市院合作项目(No.22SH13)和吉林省发改委省预算内基本建设资金项目(No.2023C042-5)资助. Supported by the National Natural Science Foundation of China(Nos.21874129,22322410),the Key Research and Development Projects of Jilin Scientific and Technological Development Program(No.202102041266YY),the Cooperation Funding of Changchun with Chinese Academy of Sciences(No.22SH13)and the Capital Construction Fund Projects within the Budget of Jilin Province(No.2023C042-5). （Nos.21874129,22322410）
