分析化学2025，Vol.53Issue(4)：602-611,10.DOI:10.19756/j.issn.0253-3820.251030
多金属纳米簇双模态磁共振成像造影剂用于前列腺癌的靶向诊断
Dual-modal Magnetic Resonance Imaging Contrast Agents Based on Polymetallic Nanoclusters for Targeted Diagnosis of Prostate Cancer
摘要
Abstract
Fe/Mn/Gd polymetallic nanooxide(FMGN)were prepared by one-step solvent thermal reaction by using Fe(acac)3,Mn(acac)2 and Gd(acac)3 as reaction precursors.Next,hyaluronic acid(HA)was used to modify FMGN to fabricate tumor-targeting T 1-T 2 dual-mode magnetic resonance imaging(MRI)contrast agent(HA-FMGN)for accurate diagnosis of prostate cancer.The structure and morphology of FMGN were observed by transmission electron microscope(TEM).It was found that FMGN exhibited a uniform nanocluster spherical structure when the feeding ratio of iron acetylacetonate,manganese acetylacetonate,and gadolinium acetylacetonate was 3:2:1.X-ray diffraction(XRD)analysis showed that FMGN had a typical inverse spinel structure of Mn doped Fe 3O 4,with Gd existing in the form of amorphous gadolinium oxide.The longitudinal relaxivity(r 1)and transverse relaxivity(r 2)of FMGN were 13.395 and 428.535 L/(mmol·s),respectively,measured by 0.5 T MRI analyzer,which proved that FMGN had excellent T 1-T 2 dual-mode MRI contrast capability.The cytotoxicity and hemolysis test found that HA-FMGN didn't damage red cells and induce toxicity for normal cells,indicating that HA-FMGN had excellent cell biocompatibility.The internalization efficacy of HA-FMGN was observed by CLSM,and the results showed that HA-FMGN possessed excellent prostate tumor-targeting ability.In vivo MRI experiment showed that HA-FMGN significantly enhanced T 1 and T 2 weighted MRI signal to noise ratio(SNR)of prostate tumor,which promoted the accurate diagnosis of orthotopic prostate cancer.关键词
磁共振成像/前列腺癌/双模态造影剂/靶向诊断Key words
Magnetic resonance imaging/Prostate cancer/Dual-mode contrast agent/Targeted diagnosis引用本文复制引用
李庆东,王鹏,肖建敏,高文娟,夏振红,张桂龙,吴正岩..多金属纳米簇双模态磁共振成像造影剂用于前列腺癌的靶向诊断[J].分析化学,2025,53(4):602-611,10.基金项目
山东省重点研发计划项目(No.2023CXPT012)和泰山学者人才工程项目(No.tsqn201909144)资助. Supported by the Key R&D Program of Shandong Province,China(No.2023CXPT012)and the Taishan Scholars Construction Engineering(No.tsqn201909144). （No.2023CXPT012）
