南方农业学报2025，Vol.56Issue(3)：911-920,10.DOI:10.3969/j.issn.2095-1191.2025.03.021
基于代谢组学探究赖氨酸水平对贵州黄鸡采食中枢差异代谢物的影响
Effects of lysine levels on differential metabolites in hypotha-lamic feeding center of Guizhou yellow chicken based on metabolomics
摘要
Abstract
[Objective]Based on metabolomics,the effects of lysine(Lys)levels on the differential metabolites of Guizhou yellow chicken's feeding center was explored,which could provide theoretical basis for exploring the optimal amount of Lys added to the diet of Guizhou yellow chicken.[Method]A total of 400 healthy 1-day-old Guizhou yellow chickens were selected and randomly divided into 4 groups.Four corn-soybean meal diets with different Lys levels(0.7%,0.9%,1.1%and 1.3%)were set up and fed to the 4 groups of Guizhou yellow chickens respectively.The body weight and feed intake were recorded.The experiment ended at 6 weeks of age.Hypothalamic tissues of Guizhou yellow chickens in the 0.7%,0.9%,and 1.3%groups were collected for liquid chromatography-mass spectrometry(LC-MS)analysis.[Result]The average daily feed intake(ADFI)of Guizhou yellow chickens aged 0-6 weeks was the highest in the 0.9%group and the lowest in the 1.3%group,and the 0.7%,0.9%and 1.1%groups were significantly higher than the 1.3%group(P<0.01,the same below).The average daily weight gain(ADG)of Guizhou yellow chickens aged 0-6 weeks was the highest in the 0.9%group and the lowest in the 1.3%group,and the 0.9%and 1.1%groups were signifi-cantly higher than the 0.7%and 1.3%groups.A total of 18,25 and 29 differential metabolites were screened out in the 0.7%vs 0.9%group,the 0.9%vs 1.3%group and the 0.7%vs 1.3%group respectively.In the 0.7%vs 0.9%group and the 0.9%vs 1.3%group,N-(2-furoyl)glycine and D-mannose were significantly different(P<0.05,the same below).D-mannose was up-regulated in the 0.7%vs 0.9%group and down-regulated in the 0.9%vs 1.3%group.KEGG signaling pathway enrichment results showed that in the 0.7%vs 0.9%group,the significantly enriched signaling pathways were glutathione metabolism,taurine and hypotaurine metabolism,D-glutamine and D-glutamate metabolism,and β-alanine metabolism.In the 0.9%vs 1.3%group,the significantly enriched signaling pathways were ABC transporters and aminoacyl-tRNA biosynthesis.In the 0.7%vs 1.3%group,the significantly enriched signaling pathways were ferroptosis,glutathione metabolism,thyroid hormone synthesis,neomycin,kanamycin and gentamicin biosynthesis.[Conclusion]Appropriate Lys levels activate signaling pathways such as glutathione metabolism and ABC transporters.Lys may affect the feeding behavior of Guizhou yellow chickens by regulating immunity,anti-inflammation and redox balance.The dy-namic changes of metabolites such as D-mannose show a close connection between nutritional signals and neural regula-tion.Lys may participate in protein synthesis or play a regulatory role in central nervous metabolism.关键词
贵州黄鸡/赖氨酸/平均日采食量/代谢组学/D-甘露糖Key words
Guizhou yellow chicken/lysine/average daily feed intake/metabolomics/D-mannose分类
农业科技引用本文复制引用
蒋耀洲,陈颖,饶永超,田应平,王小亚,吴胜,刘沁松,施晓丽,张福平..基于代谢组学探究赖氨酸水平对贵州黄鸡采食中枢差异代谢物的影响[J].南方农业学报,2025,56(3):911-920,10.基金项目
贵州省科技计划项目(黔科合支撑[2022]重点2288) （黔科合支撑[2022]重点2288）
贵州省教育厅高校自然科学研究项目(黔教技[2022]061) （黔教技[2022]061）
贵州大学引进人才科研项目(贵大人基字[2021]80号) （贵大人基字[2021]80号）
贵州农业职业学院院级科研项目(yk230106) Guizhou Science and Technology Plan Project(QKHZC[2022]Zhongdian 228) （yk230106）
Natural Science Project of the Colleges and Universities of Guizhou Department of Education(Qianjiaoji[2022]061) （Qianjiaoji[2022]061）
Research Project for Talent Introduction of Guizhou University(GDRJZ[2021]80) （GDRJZ[2021]80）
Institutional Level Research Project of Guizhou Voca-tional College of Agriculture(yk230106) （yk230106）
