南方农业学报2025，Vol.56Issue(3)：921-931,11.DOI:10.3969/j.issn.2095-1191.2025.03.022
罗汉果甜苷V增强黄牛8-细胞期胚胎发育能力的作用机制
Mechanism of mogroside V enhancing developmental ability of cattle 8-cell embryos
摘要
Abstract
[Objective]To explore the mechanism of action of mogroside V(MV)in enhancing the developmental ability of cattle 8-cell embryos,which could provide theoretical basis for improving the developmental ability of cattle embryos and improving the quality of in vitro cultured embryos using plant extracts.[Method]Cattle 4/8/16-cell embryos were collected,and transcriptome sequencing was performed using the Smart-seq2 method to explore the transcriptional expression profile of mitochondrial-related pathways in cattle embryos,and mitochondrial staining verification,uniform manifold approximation and projection(UMAP)clustering and gene set enrichment analysis(GSEA)were conducted.Different concentrations(25,50,100 μmol/L)of MV were added during the in vitro culture of cattle 8-cell embryos,and the optimal MV concentration was screened by counting the 8-cell rate and blastocyst rate.Two treatment groups were set up,normal cattle embryos(CON)and the optimal MV concentration treatment was as experimental group(MV),and immunofluorescence staining was used to detect mitochondrial function,ROS level,cell apoptosis level and DNA damage level in cattle 8-cell embryos.[Result]GSEA results showed that there were differences in the transcriptional ex-pression profiles of mitochondrial-related pathways in cattle embryos at different developmental stages.Compared with the 8-cell embryos,the mitochondrial proteins and their complex pathways in the 4-cell and 16-cell embryos were signifi-cantly up-regulated(P<0.05,the same below).Mitochondrial staining results showed that the mitochondrial fluorescence intensity of cattle 8-cell embryos was significantly lower than that at the 4-cell stage(P<0.01,the same below)and sig-nificantly lower than that at the 16-cell stage.Adding 50 μmol/L MV had the most significant effect on improving the de-velopmental ability of cattle embryos.Compared with the CON group,the mitochondrial fluorescence intensity of cattle 8-cell embryos in the MV group was significantly increased,the ROS level was significantly decreased,the fluorescence intensity of Caspase-3 was significantly decreased,and the fluorescence intensity of γ-H2AX was significantly decreased.[Conclusion]Compared with cattle 4-cell and 16-cell embryos,mitochondrial function in 8-cell embryos is reduced.Du-ring embryonic development,the addition of MV can enhance mitochondrial function and reduce oxidative stress,apopto-sis and DNA damage levels in 8-cell embryos,thereby improving the embryonic development ability of cattle and allevia-ting the developmental block of 8-cell embryos.关键词
黄牛8-细胞期胚胎/罗汉果甜苷V/线粒体/氧化应激/凋亡Key words
cattle 8-cell embryo/mogroside V/mitochondria/oxidative stress/apoptosis分类
农业科技引用本文复制引用
黄依林,尚江华,肖鹏,李孟琪,杨春艳,郑海英,李楠楠,田新如,张博,杨小淦..罗汉果甜苷V增强黄牛8-细胞期胚胎发育能力的作用机制[J].南方农业学报,2025,56(3):921-931,11.基金项目
国家自然科学基金项目(31872350) （31872350）
中央引导地方科技发展资金项目(桂科ZY24212019) （桂科ZY24212019）
国家现代农业产业技术体系广西奶水牛产业创新团队项目(nycytxgxcxtd-2021-21-01) （nycytxgxcxtd-2021-21-01）
南宁市创新创业领军人才"邕江计划"资助项目(2022004) National Natural Science Foundation of China(31872350) （2022004）
Central Government Guiding Local Science and Technology Development Project(Guike ZY24212019) （Guike ZY24212019）
Guangxi Buffalo Industry Innovation Team of China Agriculture Research System(nycytxgxcxtd-2021-21-01) （nycytxgxcxtd-2021-21-01）
Nanning Leading Talents of Innovation and Entrepreneurship"Yongjiang Plan"Funded Project(2022004) （2022004）
