贵州农业科学2025，Vol.53Issue(4)：125-132,8.DOI:10.3969/j.issn.1001-3601.2025.04.014
基于流式细胞术和基因组Survey测定川滇无患子基因组大小
Analysis on Genome Size of Sapindus delavayi Based on Flow Cytometry and Genome Survey
摘要
Abstract
[Objective]The genome size of Sapindus delavayi was determined to provide the important basis for the whole genome sequencing of S.delavayi and the genome size evaluation of other species in Sapindus.[Method]The genomic features of S.delavayi was evaluated by flow cytometry and K-mer analysis prior to a comprehensive and in-depth genome sequencing.[Result]The genome size of S.delavayi was estimated to be 450 Mb by flow cytometry.K-mer analysis predicted that the genome size of S.delavayi was 331.04 Mb,with a heterozygosity of 1.67%and the repetitive sequences accounting for approximately 40.0%.The GC content was about 36.57%.Its genome was a diploid genome with low repetition and high heterozygosity.[Conclusion]The genome of S.delavayi is a diploid genome with low repetition and high heterozygosity.关键词
川滇无患子/基因组/流式细胞术/基因组Survey/杂合度/序列Key words
Sapindus delavayi/genome/flow cytometry/genome survey/heterozygosity/sequence分类
农业科技引用本文复制引用
尹月,周美君,张永洪..基于流式细胞术和基因组Survey测定川滇无患子基因组大小[J].贵州农业科学,2025,53(4):125-132,8.基金项目
国家自然科学基金项目(31760048) （31760048）
