化工进展2025，Vol.44Issue(4)：2338-2351,14.DOI:10.16085/j.issn.1000-6613.2024-0512
硫酸盐还原菌群的构建及其在酸胁迫条件下的响应
Construction of sulfate-reducing bacteria community and its response to acid stress
摘要
Abstract
The decrease of environmental pH will affect the biochemical performance of sulfate reduction system,thus enhancing the tolerance of the system under acid stress condition has received extensive attention.In this study,an efficient sulfate-reducing bacteria(SRBs)community was constructed.Using the pure culture system inoculated with Desulfovibrio as the control group,batch experiments were carried out to investigate the effects of pH condition on the microbial growth and sulfate reduction performance of the systems,and to elucidate the physiological responses of the system microorganisms to acid stress.The results showed that the growth of bacteria and sulfate reduction reaction in the pure culture system were significantly inhibited by the decrease of pH.When the pH dropped to 5.0,the bacterial survival rate of the system was less than 10％,and the system lost its sulfate reduction function essentially.In contrast,the microorganisms in the SRBs system could improve the system resistance to acid stress through increasing the activity of ATP hydrolysis enzyme(H+-ATPase),producing stress proteins,adjusting the composition and distribution of fatty acids in the cell membrane,etc.After a 7d growth adaptation period,the SRBs system achieved sulfate reduction attaining a SO 2-4 removal rate of 30.90％and showed certain advantages in acid resistance.Furthermore,high-throughput sequencing technology was employed to analyze the response of the microbial community in the SRBs system to pH changes.It was found that acid stress had no significant effects on the α diversity of the microbial community in the SRBs system(p>0.05),but it altered the composition of the microbial community structure.The relative abundances of Bacillus and Clostridium increased significantly(26.26％and 5.14％,respectively),becoming the dominant bacterial genera.The research results revealed the adaptive regulatory mechanism of the SRBs system in weak acid environments,thereby providing a theoretical reference for the stable operation of biochemical systems under low pH conditions.关键词
硫酸盐还原/酸胁迫/菌群/响应Key words
sulfate reduction/acid stress/bacteria community/response分类
资源环境引用本文复制引用
张艳梅,李江,袁涛,刘亚洁,孙占学..硫酸盐还原菌群的构建及其在酸胁迫条件下的响应[J].化工进展,2025,44(4):2338-2351,14.基金项目
国防科技工业局核设施退役及放射性废物治理科研项目(G20210004) （G20210004）
国家自然科学基金(41772266). （41772266）
