西部中医药2025，Vol.38Issue(4)：33-41,9.DOI:10.12174/j.issn.2096-9600.2025.04.08
基于网络药理学及细胞实验探析乌附理中汤治疗胃癌的作用机制
Exploration into the Mechanism of Wufu Lizhong Tang in the Treatment of Gastric Cancer Based on Network Pharmacology and Cellular Experiment
摘要
Abstract
Objective:To discuss the mechanism of Wufu Lizhong Tang in the treatment of gastric cancer using network pharmacology and cellular experiment.Methods:The active ingredients,core targets and signaling pathways of the decoction were predicted via network pharmacology,gastric cancer cells HGC27 cells,MKN28 cells were applied to verify the main targets predicted by network pharmacology via CCK-8 experiment,RT-qPCR experiment,Western blot experiment and cellular scratch wound assay,so as to survey the mechanism of Wufu Lizhong Tang in the treatment of gastric cancer.Results:The decoction contained 198 active ingredients and 884 corresponding targets;A total of 919 differential genes associated with gastric cancer were retrieved from GEO database,and 61 intersecting targets were obtained,among them,MMP9,OLR1,PTGS2,MMP12,IL-6,SELE,MMP7 and NOX4 were the core targets;Enrichment analysis revealed that the intersecting targets were mainly enriched in PI3K/AKT,AGE-RAGE,TNF,cAMP,NF-kappa B,IL-17 and cGMP-PKG.CCK-8 and cellular scratch wound assay suggested that the decoction could obviously restrain the proliferation,migration and invasion of HGC27 cells and MKN28 cells(P<0.05).The results of RT-qPCR experiment demonstrated that the decoction could lower the expressions of SLUG,MMP9,Vimentin and VEGF mRNA(P<0.05).The results of Western blot experiment showed that the decoction could reduce the levels of p-AKT,SLUG,MMP9,Vimentin and VEGF protein(P<0.05).Conclusion:Wufu Lizhong Tang could inhibit gastric cancer through restraining AKT/GSK-3β/Snail(Slug)signaling pathway.关键词
胃癌/乌附理中汤/网络药理学/AKT/GSK-3β/Snail(Slug)信号通路Key words
gastric cancer/Wufu Lizhong Tang/network pharmacology/AKT/GSK-3β/Snail(Slug)signaling pathway分类
医药卫生引用本文复制引用
马祺,杨斌锋,乔玉洁,黄邦荣..基于网络药理学及细胞实验探析乌附理中汤治疗胃癌的作用机制[J].西部中医药,2025,38(4):33-41,9.基金项目
甘肃省自然科学基金(21JR1RA054) （21JR1RA054）
甘肃省科技计划项目(18JR2FA001) （18JR2FA001）
省级区域中医(专项)诊疗项目——肿瘤多学科诊疗中心. （专项）
