西部中医药2025，Vol.38Issue(4)：47-52,6.DOI:10.12174/j.issn.2096-9600.2025.04.10
基于网络药理学探讨丹参-红花药对治疗糖尿病周围神经病变的作用机制
Network Pharmacology-based Exploration into the Mechanism of Danshen-Honghua Couplet Medicines in the Treatment of Diabetic Peripheral Neuropathy
摘要
Abstract
Objective:To discuss the mechanism of Danshen(Salviae miltiorrhizae radix et rhizoma)-Honghua(Carthami flos)couplet medicines in the treatment of diabetic peripheral neuropathy(DPN)using network pharma-cology.Methods:The active ingredients and the corresponding targets of the two drugs were searched from TCMSP,and the standardization of the targets were performed via UniProt database;DPN-related targets were gained from GeneCards,Disgenet,TTD and OMIM,the intersecting targets of medicine and disease were screened utilizing R language;protein-protein interactions(PPI)network analysis of the intersection targets was conducted via STRING database,GO enrichment analysis and KEGG enrichment analysis of the intersection targets were performed via Bioconductor platform;Cytoscape software was utilized to construct visual control network and PPI regulation network of"active ingredients-disease targets"of the couplet medicines in the treatment of DPN,the core active ingredients and the targets of action of the core regulatory network were screened and extracted by CytoNCA plug-in.Results:The study has gained 73 active ingredients related to the couplet medicines,corresponding to 215 targets of action,among them,there were 96 targets intersected with disease;16 core active ingredients including quercetin,kaempferol,luteolin,tanshinone II A and others were screened,and there were 20 core targets of action containing AKT1,IL-1β and others;GO functional enrichment analysis involved 2497 items,mainly affecting the regulation of transcription factor activity,enzyme activity,receptor activity and biochemical processes,KEGG pathway enrichment analysis has obtained 158 pathways,involving signaling pathways such as AGE-RAGE,IL-17 and TNF in diabetic complications.Conclusion:Danshen-Honghua couplet medicines acts on the targets including AKT1 and others by intervention on AGE-RAGE signaling pathways via the active ingredients containing quercetin,kaempferol,luteolin,tanshinone II A and others,thereby treating DPN.关键词
糖尿病周围神经病变/丹参/红花/网络药理学Key words
diabetic peripheral neuropathy/Danshen/Honghua/network pharmacology分类
医药卫生引用本文复制引用
陈方飞,刘雅玲,罗幸,麦小丽,邓小敏..基于网络药理学探讨丹参-红花药对治疗糖尿病周围神经病变的作用机制[J].西部中医药,2025,38(4):47-52,6.基金项目
广西医疗卫生适宜技术开发与推广应用项目(S2018105) （S2018105）
广西中医药大学自然科学基金面上项目(2020MS019). （2020MS019）
