摘要
Abstract
Objective To investigate the effect of a curcumin-loaded core-shell structured nanofiber membrane on alveolar bone resorption in a rat model of experimental periodontitis.Methods Sixty 7-week-old SPF-grade Sprague-Dawley(SD)rats were randomly and evenly allocated to six groups(n=10 per group):control group,model group,and experimental groups 1 through 4.Rats in the control group received no intervention.Periodontitis was induced in the model group using the ligature method to establish the experimental model.Experimental groups 1 to 4 were treated with curcumin-loaded core-shell nanofiber membranes at different curcumin concentrations(0,5,10,and 20 μM/L,respectively)based on the model group.At various time points,serum inflammatory cytokine levels,the distance from the cementoenamel junction(CEJ)to the alveolar bone crest(ABC),and the volume of alveolar bone resorption were measured.Relative expression lev-els of receptor activators of nuclear factor-κB ligand(RANKL)and osteoprotegerin(OPG)proteins were also assessed.Re-sults On days 14 and 30,serum levels of IL-6,TNF-α,and IL-1β,alveolar bone resorption volume,and CEJ-ABC distance were significantly higher in the model group,experimental group 1,and experimental group 2 than in the control group and experimental group 4(P<0.05).On day 14,serum levels of IL-6,TNF-α,and IL-1β in experimental group 4 were signifi-cantly higher than those in the control group(P<0.05).On days 14 and 30,expression levels of RANKL and OPG pro-teins were significantly higher in the experimental group 1 and model group than in the control group and experimental group 4(P<0.05).On day 14,experimental group 4 also exhibited higher RANKL and OPG expression than the control group(P<0.05).Conclusion Curcumin-loaded core-shell nanofiber membranes downregulated the expression of OPG and RANKL proteins,inhibited the inflammatory response,and suppressed alveolar bone resorption in rats with periodontitis,thereby preventing further bone loss.These findings suggest that this approach may have potential clinical value in treating periodontitis.关键词
负载/姜黄素/核壳结构纳米纤维膜/大鼠/牙周炎/牙槽骨吸收Key words
Curcumin-loaded/Core-shell nanofiber membrane/Rat/Periodontitis/Alveolar bone resorption分类
医药卫生
