工业微生物2025，Vol.55Issue(2)：56-58,3.DOI:10.3969/j.issn.1001-6678.2025.02.019
抗VEGF单克隆抗体表达细胞培养工艺的优化研究
Optimization of Cell Culture Process for Anti-VEGF Monoclonal Antibody Production
吴凡 1谢钰珍 1胡海艳 1吴尧2
作者信息
- 1. 苏州工业园区服务外包职业学院,江苏 苏州 215000
- 2. 苏州晶睿生物科技有限公司,江苏 苏州 215000
- 折叠
摘要
Abstract
The objective of the study is to optimize the cell culture process for anti-vascular endothelial growth factor(VEGF)monoclonal antibodies to enhance the yield and purity of the antibodies.It screened for CHO cell lines that highly express anti-VEGF monoclonal antibodies,and optimized the medium composition and feeding strategies at both the shake flask level and the bioreactor level to achieve an increase in yield and purity.The experimental results demonstrated that the combination of Dynamis basal medium and Efficient Feed C+feed medium,along with a continuous feeding culture method,could achieve the highest monoclonal antibody yield(4.02 g/L)and purity(96.8%).The study provided important experimental data and a theoretical foundation for the process development of anti-VEGF monoclonal antibody biosimilar drugs.关键词
VEGF单克隆抗体/细胞培养工艺/培养基优化/蛋白产量/蛋白纯度Key words
VEGF monoclonal antibody/cell culture process/medium optimization/feeding strategy/protein yield/protein purity引用本文复制引用
吴凡,谢钰珍,胡海艳,吴尧..抗VEGF单克隆抗体表达细胞培养工艺的优化研究[J].工业微生物,2025,55(2):56-58,3.
