中医药导报2025，Vol.31Issue(4)：8-13,20,7.DOI:10.13862/j.cn43-1446/r.2025.04.002
黄芪多糖促进T细胞介导的抗肿瘤免疫功能研究
The Study of Astragalus Polysaccharides Promoting T Cell-Mediated Anti-Tumor Immune Function
摘要
Abstract
Objective:To investigate the anti-tumor immune-enhancing effect of Astragalus polysaccharide(APS)on T cell-mediated immunity.Methods:The LLC-GFP fluorescent cell line was constructed by trans-fecting Lewis lung cancer cells(LLC)with green fluorescent protein(GFP)through lentiviral reagent transfection to bring green fluorescence to LLC cells.The spleen of C57BL/6 hormonal mice was taken,and the primary T cells in stable state were cultured in vitro.The green fluorescence mode of the live cell analysis system was used to investigate the enhancement of T cell killing ability by Astragalus polysaccharide,with the average fluorescence intensity of LLC-GFP cells as an index.The effects of Astragali polysaccharides on the proliferation and phenotypic transformation of primary T cells were analyzed by IncuCyteR Live Cell Analysis System and flow cytometry,respectively.Results:The results of cell killing experiments showed that compared with the blank group,the fluorescence intensity of the control group and the administered group decreased gradually with the increase of time,and that APS group(40 μg/mL)showed the lowest average fluorescence intensity,which indicated that APS could enhance the killing ability of T cells against lung cancer cells.The proliferation assay showed that APS could promote the proliferation of primary T cells with the concentration at 10,20 and 40 μg/mL.The cell phenotype assay showed that the proportion of CD8+cells in primary T cells was 40.5%in the APS group(40 μg/mL),which was higher than that in the control group(37.6%).It indicated that APS played a role in immunomodulation.Conclusion:Astragalus polysaccharide can enhance the anti-tumor immunity of the body and achieve anti-tumor effects by increasing the ability of T cells to kill lung cancer cells.关键词
肺癌/黄芪多糖/T细胞/免疫增强/抗肿瘤Key words
lung cancer/Astragalus polysaccharide/T cells/immune enhancement/anti-tumor分类
医药卫生引用本文复制引用
张海桐,李鹏跃,郭子硕,杨凯丽,王晓静,陈宛灵,李加玲,阮明月,王一帆,杜守颖..黄芪多糖促进T细胞介导的抗肿瘤免疫功能研究[J].中医药导报,2025,31(4):8-13,20,7.基金项目
国家自然科学基金项目(82274111) （82274111）
