中医药导报2025，Vol.31Issue(4)：14-20,7.DOI:10.13862/j.cn43-1446/r.2025.04.003
大黄(庶)虫丸对宫颈癌细胞自噬与凋亡的作用及机制研究
The Effect of Dahuang Zhechong Wan on Autophagy and Apoptosis of Cervical Cancer Cells
摘要
Abstract
Objective:To investigate the mechanism of Dahuang Zhichong Wan(DHZCW)in inducing autophagy and apoptosis in cervical cancer cells.Methods:Network pharmacology was used to predict the targets and signaling pathways of DHZCW in the treatment of cervical cancer,and they were validated through cellular experiments.Hela cells were divided into blank control group,low dose group,medium dose group,high dose group and cisplatin group according to different experimental purposes.CCK-8 assay,scratch test,JC-1 staining,flow cytometry,transmission electron microscopy,and protein immunoblotting were applied to detect the effect and mechanism of DHZCW on autophagy and apoptosis of cervical cancer cells.Results:(1)Network pharma-cology:DHZCW had 90 core targets in cervical cancer,with 10 core targets.(2)Cellular experiments:Compared with the blank control group,low,medium,and high dose groups inhibited the proliferation and viability of Hela cells and impaired cell migration.They could induce a decrease in mitochondrial membrane potential and promote cell apoptosis.With the increase in the dose of DHZCW,there was a significant change in the morphology of mitochondria,from normal mitochondria to large swollen ones with fewer cristae,until the appearance of broken mitochondrial membrane,vacuolization,and apoptotic bodies.Compared with the blank control group,the expression levels of Beclin1 and Bcl-2 proteins decreased in low and high dose groups(P<0.05 or P<0.01),while the expression level of Bax protein increased(P<0.01).At the same time,low,medium and high dose groups showed higher Bax/Bcl-2 ratio than blank control group(P<0.05 or P<0.01).Compared with the blank control group,the expression levels of LC3B-Ⅱ/Ⅰ protein increased in low,medium and high dose groups(P<0.05 or P<0.01).Compared with the blank control group,the levels of cleaved Caspase-3 protein increased in high dose group(P<0.01).Conclusion:DHZCW can inhibit autophagy in Hela cells and promote apoptosis,with a dose-dependent effect.关键词
宫颈癌/Hela细胞/大黄(庶)虫丸/细胞自噬/细胞凋亡Key words
cervical cancer/Hela cells/Dahuang Zhechong Wan/autophagy/apoptosis分类
医药卫生引用本文复制引用
何金秀,陈乙菲,魏毅,于若男,姜德..大黄(庶)虫丸对宫颈癌细胞自噬与凋亡的作用及机制研究[J].中医药导报,2025,31(4):14-20,7.基金项目
省部共建中亚高发病成因与防治国家重点实验室开放课题(SKL-HIDCA-2021-ZY1) （SKL-HIDCA-2021-ZY1）
国家中医药管理局全国名中医周铭心传承工作室建设项目(国中医药办人教函[2018]119号) （国中医药办人教函[2018]119号）
新疆维吾尔自治区重大科技专项项目(2022A03019) （2022A03019）
