中医药导报2025，Vol.31Issue(4)：21-26,6.DOI:10.13862/j.cn43-1446/r.2025.04.004
天马颗粒剂基于miR-34a调控大肠癌细胞增殖及凋亡的实验研究
Experimental Study on the Regulation of Proliferation and Apoptosis of Colorectal Cancer Cells by Tianma Granules(天马颗粒剂)Based on miR-34a
摘要
Abstract
Objective:To investigate the molecular mechanism of Tianma granules in regulating the prolif-eration and apoptosis of colorectal cancer cells based on miR-34a.Methods:SW480 cells were incubated with drug-containing serum of Tianma granules at different concentrations.After 24 hours,MTT assay was used to screen the action concentration of drug-containing serum of Tianma granules.SW480 cells were divided into normal control group,blank serum group,normal dose group and 50%normal dose group.The SW480 cells were cultured normally in normal control group.The SW480 cells were treated with Tianma granule medicated serum(17.0 g/mL)in normal dose group,and Tianma granules medicated serum(8.5 g/mL)in 50%normal dose group.The SW480 cells were cultured with blank serum in blank serum group.After 24 hours,the expression of miR-34a was detected by RT-PCR,and the proliferation of cells was detected by MTT.The apoptosis of cells was detected by flow cytometry,and the expressions of p53 and cleaved Caspase-3 were detected by Western blotting.SW480 cells were divided into Tianma granules group,Tianma granules+NC inhibitor group,and Tianma granules+miR-34a inhibitor group.The SW480 cells were treated with drug-containing serum(17.0 g/mL)in Tianma granule group.The Tianma granule+NC inhibitor group and Tianma granule+miR-34a inhibitor group were transfected with NC inhibitor and miR-34a inhibitor,respectively,followed by intervention with drug-containing serum(17.0 g/mL)of Tianma granules.After 24 h,the expressions of p53 and cleaved Caspase-3 were detected by Western blotting.Results:The serum concentration of Tianma granules in vitro was 17.0 μg/mL.Tianma granule could inhibit the proliferation of SW480 cells,promote the apoptosis of SW480 cells,and up-regulate the expression of miR-34a,p53 and cleaved Caspase-3 in colorectal cancer cells.The regulatory effect on colorectal cancer cells was dose-dependent.Inhibition of miR-34a could reverse the pro-apoptosis and anti-proliferation effects of Tianma granules on colorectal cancer cells.Conclusion:Tianma granules can inhibit colorectal cancer cell proliferation and promote colorectal cancer cell apoptosis by up-regulating the expressions of p53 and cleaved Caspase-3 by up-regulating miR-34a.关键词
大肠癌/天马颗粒剂/miR-34a/细胞增殖/细胞凋亡Key words
colorectal cancer/Tianma granules/miR-34a/cell proliferation/cell apoptosis分类
中医学引用本文复制引用
马亚波,胡响当,鲁海燕,罗敏,徐胜艳,李磊磊,杨宗亮..天马颗粒剂基于miR-34a调控大肠癌细胞增殖及凋亡的实验研究[J].中医药导报,2025,31(4):21-26,6.基金项目
湖南中医药大学校级科研基金资助项目(2021XJJJ064) （2021XJJJ064）
湖南省自然科学基金资助项目(2022JJ30448) （2022JJ30448）
湖南中医药大学中医学一流学科开放基金项目(2022ZYX06) （2022ZYX06）
