中医药导报2025，Vol.31Issue(4)：27-33,7.DOI:10.13862/j.cn43-1446/r.2025.04.005
山楂酸对鼻咽癌细胞迁移与侵袭的干预作用研究
Study on the Intervention Effect of Maslinic Acid on the Migration and Invasion of Nasopharyngeal Carcinoma Cells
摘要
Abstract
Objective:To investigate the effects and mechanisms of maslinic acid(MA)on the migration and invasion of nasopharyngeal carcinoma(NPC)cells.Methods:NPC cell lines S18 and S26 were divided into drug groups(10,20,30,40,50,60,70,80 μmol/L MA),control group,and cisplatin(CIS)group.The MTT assay was used to evaluate the inhibitory effect of MA on NPC cell proliferation.For migration and invasion assays,S18 and S26 cells were divided into drug groups(10,15,20 μmol/L MA),control group and CIS group.Scratch wound healing and Transwell invasion chamber assays were performed to assess cell migration and invasion.For protein analysis,S18 and S26 cells were divided into drug groups(20,40 μmol/L MA),control group and CIS group.Western blotting was used to detect the expression levels of migration-related proteins(Vimentin,N-cadherin).A nude mouse footpad-inguinal lymph node metastasis model was established using NPC cells.Successfully modeled mice were randomly divided into control group,MA group and CIS group,6 mice in each group.After 20 days of drug intervention,inguinal lymph node size and weight,liver/kidney/spleen weights,and lymph node metastasis were evaluated.Results:MTT assays showed that the relative proliferation rates of S18 and S26 cells in drug groups were significantly lower than those in the control group at 36 and 48 h post-treatment(P<0.05 or P<0.01).Scratch wound healing assays demonstrated that drug groups showed lower relative migration of S18 and S26 cells than control group at 12,24,36,and 48 h post-treatment(P<0.05 or P<0.01).Transwell assays demonstrated that drug groups(15,20 μmol/L)showed lower relative invasion rates of S18 cells than control group(P<0.01),and that drug groups showed lower relative invasion rates of S26 cells than control group(P<0.05).Western blotting demonstrated that drug groups(S18,S26)showed lower Vimentin and N-cadherin protein expression(P<0.05).Animal experiments showed that MA group and CIS group showed lower lymph node metastasis rates than control group(P<0.05),with lower lymph node/liver/spleen weights than control group(P<0.05).MA group showed fewer metastatic foci(HE staining)than control group.Conclusion:Maslinic acid can inhibit NPC cell proliferation,migration and invasion related protein expression.关键词
鼻咽癌/山楂酸/细胞迁移/细胞侵袭/上皮间质转化Key words
nasopharyngeal carcinoma/maslinic acid/cell migration/cell invasion/epithelial-mesenchymal transition分类
中医学引用本文复制引用
袁悦萌,陈姝霖,王晓娟,王雯,吴仪彬,何迎春,周芳亮..山楂酸对鼻咽癌细胞迁移与侵袭的干预作用研究[J].中医药导报,2025,31(4):27-33,7.基金项目
湖南省大学生创新创业训练计划项目(2023-2335) （2023-2335）
湖南省科技创新计划项目(2024RC3202) （2024RC3202）
