中医药导报2025，Vol.31Issue(4)：34-41,8.DOI:10.13862/j.cn43-1446/r.2025.04.006
菊藻丸对乳腺癌MCF-7细胞PTEN/PI3K/Akt/mTOR信号通路的影响
The Influence of Juzao Pill(菊藻丸)on the PTEN/PI3K/Akt/mTOR Signaling Pathway in MCF-7 Breast Cancer Cells
摘要
Abstract
Objective:To investigate the effects and mechanisms of Juzao Pill-containing serum on inhibiting the malignant behaviors of human breast cancer MCF-7 cells through regulating the PTEN/PI3K/Akt/mTOR signaling pathway.Methods:shRNA targeting human PTEN gene and negative control NC sequences were designed and synthesized.Lentiviral vectors for PTEN gene silencing were constructed and used to infect MCF-7 cells.The knockdown effect of PTEN was verified by Real-time PCR and Western blotting.Cells were divided into control group,blank serum group,Juzao Pill-containing serum group,LV-shNC group,LV-shNC+Juzao Pill-containing serum group,LV-shPTEN group,LV-shPTEN+Juzao Pill-containing serum group,and LV-shPTEN+Juzao Pill-containing serum+MK2206 group.Cell proliferation was detected by CCK-8 and colony formation assays.Apoptosis level was examined by TUNEL staining.Cell cycle distribution was analyzed by flow cytometry.Scratch wound healing and Transwell assays were performed to assess cell migration and invasion abilities,respectively.Key protein expression changes in the PTEN/PI3K/Akt/mTOR pathway were detected by Western blotting.Results:Real-time PCR and Western blotting confirmed that PTEN mRNA and protein expression levels were significantly reduced after PTEN shRNA transfection in MCF-7 cells.Compared with the blank serum group,the Juzao Pill-containing serum group showed significantly decreased proliferation,colony formation,migration and invasion abilities,increased apoptosis level,more obvious G1 phase arrest,upregulated PTEN expression,and reduced phosphorylation levels of p-PI3K,p-Akt,and p-mTOR(P<0.05).There was no statistical difference in each indicator between the LV-shNC group and the blank serum group(P>0.05).Compared with the LV-shNC group,the LV-shNC+Juzao Pill-containing serum group exhibited inhibited cell proliferation,colony formation,migration and invasion abilities,increased apoptosis rate and G1 phase proportion,upregulated PTEN expression,and decreased PI3K/Akt/mTOR pathway activity(P<0.05).Compared with the LV-shNC+Juzao Pill-containing serum group,the LV-shPTEN+Juzao Pill-containing serum group showed enhanced cell proliferation,invasion and metastasis abilities,decreased apoptosis level and G1 phase proportion,reduced PTEN expression,and hyperactivated PI3K/Akt/mTOR pathway(P<0.05).The LV-shPTEN+Juzao Pill-containing serum+MK2206 group exhibited more obvious suppression of malignant phenotypes and further reduced PI3K/Akt/mTOR pathway activity compared with the LV-shPTEN+Juzao Pill-containing serum group(P<0.05).Conclusion:Juzao Pill-containing serum can inhibit the proliferation,invasion and metastasis,and promote the apoptosis and G1 phase arrest of breast cancer MCF-7 cells by activating PTEN and inhibiting the PI3K/Akt/mTOR signaling pathway,which may be one of the important mechanisms of Juzao Pill in exerting anti-breast cancer effects.关键词
乳腺癌/菊藻丸/PTEN/PI3K/Akt/mTOR/细胞增殖/侵袭转移/凋亡/细胞周期Key words
breast cancer/Juzao Pill/PTEN/PI3K/Akt/mTOR/cell proliferation/invasion and metastasis/apoptosis/cell cycle分类
中医学引用本文复制引用
欧阳志,郑裴凡,李松莲..菊藻丸对乳腺癌MCF-7细胞PTEN/PI3K/Akt/mTOR信号通路的影响[J].中医药导报,2025,31(4):34-41,8.基金项目
湖南省自然科学基金科药联合项目(2022JJ80022) （2022JJ80022）
