中医药导报2025，Vol.31Issue(4)：48-52,5.DOI:10.13862/j.cn43-1446/r.2025.04.008
蒲黄多糖调控YAP/TAZ通路对四氯化碳诱导的小鼠肝纤维化的影响
Effects of Pollen Typhae Polysaccharide on Carbon Tetrachloride Induced Liver Fibrosis in Mice by Regulating YAP/TAZ Pathway
摘要
Abstract
Objective:To investigate the effect of Pollen typhae polysaccharide on carbon tetrachloride-induced liver fibrosis in mice.Methods:Totally 40 mice were randomly divided into 4 groups,including control group,model group,Pollen typhae polysaccharide group and verteporfin group.Carbon tetrachloride was used to induce liver fibrosis in mice.The mice were gavaged with Pollen typhae polysaccharide(50 mg/kg)per day in the Pollen typhae polysaccharide group.Mice were given normal saline by gavage in the control group and model group.Mice were given verteporfin(5 mg/kg)via tail vein every day in verteporfin group.Each group received continuous intervention for 4 weeks.Hematoxylin-eosin staining was used to analyze the pathological changes of the liver.Masson staining was used to analyze liver fibrosis in each group.The levels of liver function and inflammatory factors in serum of mice in each group were analyzed by enzyme-linked immunosorbent assay.The expression of Yes-associated protein pathway proteins was analyzed by Western blotting.Results:The liver tissue of the model group showed severe pathological damage,while the liver tissue damage of the Pollen typhae polysaccharide group and verteporfin group was improved.Compared with the model group,the area of liver fibrosis was decreased in the Pollen typhae polysaccharide group and verteporfin group(P<0.05).Compared with the model group,the liver function indexes were improved in Pollen typhae polysaccharide group and verteporfin group,and the levels of inflammatory factors were decreased(P<0.05).Compared with the model group,the expression of YAP,TAZ and CTGF in the liver was inhibited in Typha polysaccharide group and verteporfin group(P<0.05).Conclusion:Pollen typhae polysaccharide can improve carbon tetrachloride induced liver fibrosis in mice,and this process is related to the regulation of Yes-associated protein pathway by Pollen typhae polysaccharide.关键词
蒲黄多糖/Yes相关蛋白/肝纤维化/四氯化碳/小鼠/维替泊芬/炎症Key words
Pollen typhae polysaccharide/Yes-associated protein/liver fibrosis/carbon tetrachloride/mice/verteporfin/inflammation分类
中医学引用本文复制引用
苏志威,薛娟,孙俊,朱锐,范恒,计春燕..蒲黄多糖调控YAP/TAZ通路对四氯化碳诱导的小鼠肝纤维化的影响[J].中医药导报,2025,31(4):48-52,5.基金项目
湖北省卫生健康委2021-2022年度卫生健康科研立项项目(WJ2021F120) （WJ2021F120）
2023年湖北省中西医结合医院"杏林人才工程"院级科研重点项目(H2023Z005) （H2023Z005）
