中医药导报2025，Vol.31Issue(4)：53-59,7.DOI:10.13862/j.cn43-1446/r.2025.04.009
基于网络药理学预测柳蒿芽脂溶性部位的降血脂作用及实验验证
Prediction and Validation of Hypolipidemic Effect of Liposoluble Parts from Artemisia Integrifolia Based on Network Pharmacology
摘要
Abstract
Objective:To investigate the hypolipidemic effect of liposoluble parts from Artemisia integrifolia(LPAI).Methods:The target prediction of 20 chemical components collected from LPAI was carried out by Swiss Target Prediction and SEA database,and the hyperlipidemia(HLP)targets were obtained in TTD and GeneCards.The potential targets of LPAI for the treatment of HLP were obtained by using bioinformatics to match genes and draw Venn diagrams.The PPI network diagram was constructed on String website,and Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were carried out through Metascape database.The Cytoscape 3.6.0 Software was used to build the network of compounds-targets-pathways for LPAI against HLP.Then,the core targets and pathways were preliminarily verified by the in vitro experiment of L-02 cell steatosis model induced by FFA after the intervention of LPAI.The effects of LPAI on proliferation of L-02 cells,the content of cell lactone drops and triglyceride(TG)were investigated,and Western blotting was used to detect expression of peroxisome proliferator activated receptor α(PPARα)and lipoprotein lipase(LPL).Results:Totally 84 potential targets that LPAI associated with HLP were screened.The functional enrichment analysis of GO obtained 662 GO items,including 614 biological processes,31 molecular functions and 17 cellular components.KEGG pathway enrichment analysis showed that the main signal pathways of LPAI in the treatment of HLP include PPAR signal pathway,insulin resistance,adipocytokine signal pathway and so on.CCK-8 experiment showed that LPAI could reduce the proliferation of L-02 cells induced by FFA,which was dependent on dose and time.Oil red O staining experiment showed that the intracellular lipid droplets were distributed at the edge of cell membrane in LPAI low,medium and high dose groups,and that the cumulative number gradually decreased,accompanied by the improvement of different progress.The model groups showed higher TG content than control group(P<0.01).The LPAI medium and high dose groups showed lower TG content than model group(P<0.05 and P<0.01).The model group showed lower expression levels of PPAR α and LPL than control group(P<0.01 and P<0.05);The LPAI low,medium and high dose groups showed higher expression levels of LPL protein than model group(P<0.05),the LPAI medium dose group showed higher expression of PPAR α protein than model group(P<0.05).Conclusion:LPAI can reduce blood lipid and inhibit the expression of PPAR α and LPL in FFA-induced L-02 cells by regulating PPAR signal pathway.关键词
高脂血症/柳蒿芽/脂溶性部位/网络药理学/降血脂Key words
hyperlipidemia/Artemisia integrifolia/liposoluble parts/network pharmacology/hypolipidemic effect分类
中医学引用本文复制引用
明慧,王青虎,何祥,伊力奇..基于网络药理学预测柳蒿芽脂溶性部位的降血脂作用及实验验证[J].中医药导报,2025,31(4):53-59,7.基金项目
内蒙古自治区科技计划项目(2019GG118) （2019GG118）
蒙药研发国家地方联合工程研究中心开放基金项目(MDK2023040) （MDK2023040）
