中医药导报2025，Vol.31Issue(4)：67-72,6.DOI:10.13862/j.cn43-1446/r.2025.04.011
基于P38MAPK/P53信号通路探讨寿胎丸对复发性流产小鼠的影响
The Effect of Shou Tai Wan(寿胎丸)on Recurrent Abortion Mice Based on P38MAPK/P53 Signaling Pathway
摘要
Abstract
Objective:To observe the effects of Shou Tai Wan on recurrent abortion(RSA)mice,and to study its mechanism of action through the P38MAPK/P53 signaling pathway.Methods:According to Clark's classical modeling procedure,40 female CBA/J mice and 20 male DBA/2 mice were mated in a combined cage based on a 2∶1 ratio to construct the RSA mouse model,and 40 pregnant mice were randomly divided into dydrogesterone group,model group,Shou Tai Wan high dose group,Shou Tai Wan medium dose group,and Shou Tai Wan low dose group,with 8 mice in each group.Totally 8 female CBA/J mice and 4 male BALB/c mice were caged together based on a 2∶1 ratio to construct a normal pregnancy mouse model,and the 8 normal pregnancy mice model was set as blank group.The mice in each group were given appropriate drug interventions.The pregnancy status of the mice was observed,and the embryo loss rate was calculated.Hema-toxylin-eosin(HE)staining was used to observe the histopathological changes in the placenta of the mice in each group.Western blotting detected p38 mitogen-activated protein kinase(P38MAPK),phosphorylated p38MAPK(p-P38MAPK),oncogene(P53),and B-cell lymphoma-2(Bcl-2)protein expression.ELISA was used to detect MDA content in serum.PCR was used to detect the expression of P38MAPK mRNA,p-P38MAPK mRNA,P53 mRNA,and Bcl-2 mRNA in placental tissues.Results:The uterus of mice in the blank group was light red,with balanced embryonic development,uniform size and orderly arrangement.The placenta of mice in the model group was irregularly arranged with disrupted morphology and solidified cell nuclei.Compared with the model group,the pathological state of the placenta tissue was improved in the dydrogesterone group as well as the Shou Tai Wan high,medium and low dose groups,and the cellular arrangement was orderly with a more regular morphology.The model group showed higher embryo loss rate and serum MDA content than blank group(P<0.01).The Shou Tai Wan high dose group,Shou Tai Wan medium dose group and dydrogesterone group showed lower embryo loss rate and serum MDA content than model group(P<0.01 or P<0.05).The model group showed higher p-38MAPK/P38MAPK and relative expression of P53 proteins than blank group(P<0.01),while lower relative expression of Bcl-2 proteins than blank group(P<0.01).The Shou Tai Wan high dose group,Shou Tai Wan medium dose group and dydrogesterone group showed lower p-38MAPK/P38MAPK and relative expression of P53 proteins than model group(P<0.01).The Shou Tai Wan high dose group,Shou Tai Wan medium dose group,Shou Tai Wan low dose group and dydrogesterone group showed higher relative expression of Bcl-2 proteins than model group(P<0.01).The model group showed higher relative expression of P38MAPK mRNA and P53 mRNA than blank group(P<0.01),while lower relative expression of Bcl-2 mRNA than blank group(P<0.01).The Shou Tai Wan high dose group,Shou Tai Wan medium dose group,Shou Tai Wan low dose group and dydrogesterone group showed lower relative expression of P38MAPK mRNA and P53 mRNA than model group(P<0.01 or P<0.05),while higher relative expression of Bcl-2 mRNA than model group(P<0.01).Conclusion:Shou Tai Wan may regulate oxidative stress through the P38MAPK/P53 signaling pathway,and play a role in preventing recurrent abortion in mice.关键词
复发性流产/寿胎丸/P38MAPK/P53通路/氧化应激/小鼠Key words
recurrent miscarriage/Shou Tai Wan/P38MAPK/P53 pathway/oxidative stress/mice分类
中医学引用本文复制引用
冯玮,王艳辉,史力卉,雷磊..基于P38MAPK/P53信号通路探讨寿胎丸对复发性流产小鼠的影响[J].中医药导报,2025,31(4):67-72,6.基金项目
湖南省教育厅科学研究项目(23A0306) （23A0306）
