中医药导报2025，Vol.31Issue(4)：73-76,108,5.DOI:10.13862/j.cn43-1446/r.2025.04.012
HPLC-DAD多波长切换法同时测定清肝化瘀方中9个成分含量
Simultaneous Determination of Nine Components in Qinggan Huayu Recipe(清肝化瘀方)by HPLC-DAD Multiwavelength Switching Method
摘要
Abstract
Objective:A high performance liquid chromatography-photodiode array detector(HPLC-DAD)method was used to establish a determination method for the content of multiple components of Qinggan Huayu Recipe(清肝化瘀方).Methods:Use Agela ASB C18 chromatographic column(5 μm,4.6 mm×250 mm),use acetonitrile(A)-0.4%phosphoric acid(B),gradient elution 0 to 10 minutes,5%to 15%A;10 to 20 minutes,15%to 20%A;20 to 45 min,20%to 30%A;45 to 60 min,30%to 70%A,column temperature:25℃,flow rate 1.0 mL/min,injection volume 10 μL,detection wavelengths are 210 nm(0-11 min,detect sodium danshensu),327 nm(11-24 min,detect chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid),292 nm(24-35 min,detect astilbin),286 nm(35-42 min,detect salvianolic acid B),370 nm(42-60 min,detect quercetin,kaempferol),290 nm(50-52.5 min,detect naringenin).Results:Sodium danshensu,salvianolic acid B,quercetin,kaempferol,naringenin,astilbin,chlorogenic acid,neochlorogenic acid and cryptochlorogenic acid showed good linear relationships in their respective concentration ranges,with the correlation coefficients greater than 0.999 5,and the precision,stability,and reproducibility were good,with RSDs were all<5.00%.The average recoveries(n=6)of sodium danshensu,salvianolic acid B,astilbin,chlorogenic acid,neochlorogenic acid and cryptochlorogenic acid ranged from 99.39%to 103.43%,and the RSDs were all<5%.Conclusions:The established HPLC-DAD method can simultaneously determine the contents of nine components in Qinggan Huayu Recipe,which is simple,rapid and accurate.关键词
清肝化瘀方/HPLC-DAD法/多成分测定/含量测定Key words
Qinggan Huayu Recipe/HPLC-DAD method/multicomponent determination/content determination分类
中医学引用本文复制引用
郭紫云,杜丽娜,张秋燕,谢晓暄,杨燕..HPLC-DAD多波长切换法同时测定清肝化瘀方中9个成分含量[J].中医药导报,2025,31(4):73-76,108,5.基金项目
国家自然科学基金项目(82205184) （82205184）
北京市属医院科研培育计划(PZ2022027) （PZ2022027）
