中医药导报2025，Vol.31Issue(4)：83-88,6.DOI:10.13862/j.cn43-1446/r.2025.04.014
一测多评法同时测定无敌丹胶囊中7种成分的含量
Simultaneous Determination of Seven Components in Wudidan Capsules(无敌丹胶囊)by QAMS
摘要
Abstract
Objective:To establish a quantitative analysis method of multi-components by single marker(QAMS)for simultaneous determination of pinoresinol diglucoside,calycosin glucoside,notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1,asperosaponin Ⅵ,and psoralen in Wudidan capsules.Methods:Using octadecylsilane bonded silica gel as a filler,use a WondaSil C18 Superb chromatography column(250.0mm×4.6mm,5 μm),using acetonitrile-0.05%phosphoric acid water as the mobile phase,gradient elution;volume flow rate of 1.0 mL/min;column temperature of 30℃;detection wavelength of 203 nm.Calculate the relative correction factors of the other six components using the flavonoid glucoside from Fructus Mori as the internal standard,and determine their content.Results:The seven components showed good linear relationships within their respective ranges(r≥0.9991),with an average recovery rate of 98.84%to 101.33%(RSD<3%).There was no significant difference in the content determination results between the one test multiple evaluation method and the external standard method(RSD<2%).Conclusion:The established method was accurate and reliable and could be applied for the simultaneous determination of pinoresinol diglucoside,calycosin glucoside,notogin-senoside R1,ginsenoside Rg1,asperosaponin Ⅵ,ginsenoside Rb1,and psoralen in Wudidan capsules.关键词
无敌丹胶囊/一测多评法/毛蕊异黄酮葡萄糖苷/质量控制/含量测定Key words
Wudidan capsules/QAMS/calycosin glucoside/quality control/content determination分类
社会科学引用本文复制引用
李晴红,王娟,张晓平,计飞,杨兴波,张文平..一测多评法同时测定无敌丹胶囊中7种成分的含量[J].中医药导报,2025,31(4):83-88,6.基金项目
云南省横向课题(20201120001) （20201120001）
