甘肃中医药大学学报2025，Vol.42Issue(2)：36-41,6.DOI:10.16841/j.issn1003-8450.2025.02.08
小苞黄芪多糖的分离、结构表征及抗氧化活性研究
Study on the isolation,structural characterization and antioxidant activity of polysaccharides from Astragalus prattii Simps
摘要
Abstract
Objective To study the antioxidant activity of Astragalus prattii Simps polysaccharide(APS).Methods The polysaccharides were extracted and purified through water extraction and graded alcohol precipita-tion.Four APS fractions(APS20,APS40,APS60,and APS80)obtained at ethanol concentrations of 20％,40％,60％,and 80％respectively underwent comprehensive structural characterization using multiple analytical tech-niques.The scavenging abilityof 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radicals,2,2'-diazide(3-ethylbenzothiazolin-6-sulfonic acid)diamiammonium salts(ABTS)free radicals,hydroxyl free radicals,and the che-lating ability of Fe2+were derermined.Results It demonstrated sugar contents of 63.2％,68.7％,73.4％,and 65.9％for the four APS fractions,all containing seven monosaccharide components:mannose(Man),rhamnose(Rha),glucuronic acid(GlcA),galacturonic acid(GalA),glucose(Glu),galactose(Gal),and arabinose(Ara).Molecular weight distributions ranged from 3.0×103 to 3.2×104 Da,with all samples exhibiting flaky morphology.Fourier-transform infrared spectroscopy(FT-IR)analysis confirmed the presence of uronic acids and both α-and β-configurations,while nuclear magnetic resonance(NMR)results specifically indicated β-glycosidic bond dominance in APS60.Antioxidant assays revealed superior radical scavenging capabilities across all APS fractions,particularly demonstrating significantly enhanced Fe2+chelation efficiency compared to L-ascorbic acid(VC).Conslusion These findings substantiate that APS effectively reduces free radical generation and protects cells from oxidative damage,providing experimental evidence for further development of its medicinal value.关键词
小苞黄芪/多糖/结构表征/抗氧化活性/自由基清除/Fe2+螯合Key words
Astragalus prattii Simps/polysaccharides/structural characterization/antioxidant activity/free radi-cal scavenging/Fe2+chelation分类
医药卫生引用本文复制引用
王莉,游亚,王蕊霞,段国建..小苞黄芪多糖的分离、结构表征及抗氧化活性研究[J].甘肃中医药大学学报,2025,42(2):36-41,6.基金项目
2024年甘肃省高校大学生就业创业提升工程项目(202402) （202402）
甘肃省科技计划项目(24JRRA883) （24JRRA883）
甘肃省教育厅产业支撑计划项目(2025CYZC-050). （2025CYZC-050）
