海南医科大学学报2025，Vol.31Issue(8)：570-577,8.DOI:10.13210/j.cnki.jhmu.20250407.001
PA2G4通过PI3K/AKT/p53信号通路对胆囊癌增殖、迁移及凋亡的作用机制研究
Mechanism of PA2G4 on proliferation,migration,and apoptosis of gallbladder cancer through PI3K/AKT/p53 signaling pathway
摘要
Abstract
Objective:To investigate the mechanism of proliferation-associated protein 2G4(PA2G4)on the proliferation,mi-gration,and apoptosis of human gallbladder cancer cells GBC-SD through PI3K/AKT/p53 signaling pathway.Methods:Lentivi-ral transfection technique was utilized to construct gallbladder cancer cell lines with down-regulated PA2G4 expression.RT-qPCR and Western blot were used to verify the transfection efficiency.CCK-8 assay and colony formation assay were utilized to assess the proliferative activity of PA2G4 for GBC-SD cells.Transwell assay and scratch healing assay were used to assess the effect of PA2G4 on the migration ability of GBC-SD cells.The expression levels of PA2G4 on PI3K/AKT/p53 and its related phosphory-lated proteins were detected by Western blot technique,and the expression levels of apoptosis-related proteins Caspase-3,Bcl-2 and Bax were detected.Results:The mRNA and protein expression levels of PA2G4 were significantly decreased in GBC-SD cells after PA2G4 knockdown.The knockdown of PA2G4 significantly inhibited the proliferative activity and migration ability of GBC-SD cells,while promoting apoptosis.In addition,PA2G4 knockdown was accompanied by down-regulation of p-PI3K and p-AKT expression levels,as well as up-regulation of p-p53 expression level.Conclusion:The present study demonstrates that PA2G4 can inhibit the proliferation and migration of gallbladder cancer cells and induce their apoptosis through the down-regula-tion of PI3K/AKT/p53 signaling pathway.关键词
胆囊癌/PA2G4/细胞增殖/凋亡Key words
Gallbladder cancer/PA2G4/Cell proliferation/Apoptosis分类
医药卫生引用本文复制引用
王胜威,严华悦,周汉旭,杨立春,刘子祥,王畏,贾建光..PA2G4通过PI3K/AKT/p53信号通路对胆囊癌增殖、迁移及凋亡的作用机制研究[J].海南医科大学学报,2025,31(8):570-577,8.基金项目
This study was supported by the Anhui Province Health and Wellness Key Scientific Research Program(AHWJ2023A10093),Anhui Provincial Clinical Key Specialty Construction Project Funding(AHWJ2022b079),Anhui Province Higher Education Institutions Natural Science Research Key Program(2022AH051476),Natural Science Key Program of Bengbu Medical College(2021byzd19),and Bengbu Medical College 2023 Graduate Student Research and Innovation Program(Byycx23127) 安徽省卫生健康重点科研项目(AHWJ2023A10093) （AHWJ2023A10093）
安徽省临床重点专科建设项目经费(AHWJ2022b079) （AHWJ2022b079）
安徽省高等学校自然科学研究重点项目(2022AH051476) （2022AH051476）
蚌埠医学院自然科学重点项目(2021byzd197) （2021byzd197）
蚌埠医学院 2023年度研究生科研创新计划(Byy-cx23127) （Byy-cx23127）
