黑龙江动物繁殖2025，Vol.33Issue(2)：6-10,15,6.DOI:10.19848/j.cnki.ISSN1005-2739.2025.04.0002
不同超排程序对荷斯坦奶牛性控胚胎生产的影响
Effects of different superovulation protocols on sex-controlled embryo production in Holstein dairy cows
李金泽 1王定国 2傅祥伟 3麻柱 4陈超凡 5刘爱菊 5刘成军 4李艳华 4薛建华 4李玉龙 4李俊 5赵蓉5
作者信息
- 1. 新疆农业大学动物科学学院,新疆 乌鲁木齐 830052||北京奶牛中心,北京 100192
- 2. 新疆农业大学动物科学学院,新疆 乌鲁木齐 830052
- 3. 新疆农业大学动物科学学院,新疆 乌鲁木齐 830052||中国农业大学动物科技学院,北京 100193
- 4. 北京奶牛中心,北京 100192
- 5. 中国农业大学动物科技学院,北京 100193
- 折叠
摘要
Abstract
The experiment was conducted to analyze the effects of different superovulation protocols on the production of sex-controlled embryos in Holstein dairy cows.A total of 89 healthy Holstein dairy cows aged 12 to 14 months were used as donors,in which embryos were collected in vivo using the flushing technique to compare the effects of two superovulation protocols.Protocol 1 involved the use of follicle-stimulating hormone-Ningbo(FSH-N,total dose of 500 IU),with a decreasing dose(4 mL to 1 mL)administered over 4 d,followed by the injection of human chorionic gonadotropin(hCG)on the 9th day to induce ovulation and timed insemination.Protocol 2 involved the use of follicle-stimulating hormone-Reprobiol(FSH-R,total dose of 500 IU),with a lower dose decreasing scheme(2 mL to 0.5 mL),and the insemination was carried out on the 9th day of estrus.Both protocols involved the placement of a vaginal sponge and the injection of estradiol(E2)and progesterone(P4)on the first day,the injection of prostaglandin(PG)and removal of the sponge on the 7th day,and embryo flushing was done on the 16th day.The number of embryos recovered per head,the number of high-quality embryos per head,and the rate of high-quality embryos were recorded and statistically analyzed.The results showed that protocol 2 was more effective than protocol 1 in terms of the number of embryos recovered per head(9.75±4.54 vs.4.16±2.49)and the number of high-quality embryos per head(3.96±2.65 vs.1.66±1.34,P<0.05),while there was no significant difference in the rate of high-quality embryos(40.64%vs.39.87%,P>0.05).In conclusion,under the conditions of this experiment,protocol 2(using FSH products from Reprobiol,Belgium)was more effective.关键词
荷斯坦奶牛/促卵泡素/超数排卵程序/体内胚胎生产/优质胚数Key words
Holstein dairy cows/follicle-stimulating hormone/superovulation procedure/in vivo embryo production/number of fine embryos分类
农业科技引用本文复制引用
李金泽,王定国,傅祥伟,麻柱,陈超凡,刘爱菊,刘成军,李艳华,薛建华,李玉龙,李俊,赵蓉..不同超排程序对荷斯坦奶牛性控胚胎生产的影响[J].黑龙江动物繁殖,2025,33(2):6-10,15,6.
