国际口腔医学杂志2025，Vol.52Issue(3)：349-357,9.DOI:10.7518/gjkq.2025046
沉默信息调节因子1调控成骨成血管功能促进颌骨缺损愈合的实验研究
Promotion of mandibular defect healing through the regulation of osteogenic and angiogenic functions by sirtuin 1
摘要
Abstract
Objective This study aims to investigate the effect of sirtuin 1(SIRT1)on osteogenic and angiogenic func-tions in mice under in vivo and in vitro conditions,as well as its effect on mandibular defect healing.Methods SIRT1 ac-tivators and inhibitors were used to intervene in MC3T3-E1 cells and mandibular defects in mice.Various methods,in-cluding cell counting kit-8(CCK-8)assay,real-time quantitative polymerase chain reaction,Western blot,alkaline phos-phatase staining,and immunofluorescence staining,were employed to study the influence of SIRT1 on the expres-sion of osteogenic and angiogenic factors in MC3T3-E1 cells,as well as the healing and osteogenic and angio-genic functions of mandibular defects in mice.Results In in vitro experiments,the activation of SIRT1 promo-ted the expression of osteogenic and angiogenic factors in MC3T3-E1 cells.In in vivo experiments,SIRT1 activation facilitated the hea-ling of mandibular defects and enhanced the osteogenic and angiogenic functions of the mandibular defects.Conversely,the inhibition of SIRT1 activity sup-pressed the aforementioned processes.Conclusion SIRT1 can promote the healing of mandibular defects by regulating the osteogenic and angiogenic functions in mice.关键词
沉默信息调节因子1/血管生成/颌骨缺损/骨再生Key words
sirtuin 1/angiogenesis/mandibular defect/bone regeneration分类
口腔医学引用本文复制引用
刘志凯,刘航航,刘士博,李帛伦,刘瑶,罗恩..沉默信息调节因子1调控成骨成血管功能促进颌骨缺损愈合的实验研究[J].国际口腔医学杂志,2025,52(3):349-357,9.基金项目
National Natural Science Foundation of China(82370932) （82370932）
Key Research and Development Project of Si-chuan Province(2024YFFK0204) （2024YFFK0204）
Natural Science Foundation Youth Project of Sichuan Province(2023NSFSC1512) 国家自然科学基金面上项目(82370932) （2023NSFSC1512）
四川省重点研发项目(2024YFFK0204) （2024YFFK0204）
四川省自然科学基金青年项目(2023-NSFSC1512) （2023-NSFSC1512）
