国际口腔医学杂志2025，Vol.52Issue(3)：358-365,8.DOI:10.7518/gjkq.2025045
支链氨基酸转氨酶1在口腔鳞状细胞癌中的表达及其功能研究
Expression and function of branch-chain amino acid transaminase 1 in oral squamous cell carcinoma
摘要
Abstract
Objective This study aimed to analyze the expression and biological function of branch-chain amino acid transaminase 1(BCAT1)in oral squamous cell carcinoma(OSCC).Methods The expression of BCAT1 in head and neck squamous cell carcinoma(HNSC)patients and its relationship with prognosis were analyzed by bioinformatics.To detect BCAT1 gene expression in tumor and adjacent tissues,we selected patients with OSCC diagnosed in the Depart-ment of Oral and Maxillofacial Surgery,Xiangya Stomatology Hospital,Central South University from 2021 to 2022.BCAT1 gene expression in human OSCC cell line and normal oral keratinocytes was analyzed.After plasmid overexpres-sion or knockdown of BCAT1 gene,the proliferation,migration,and invasion ability of human OSCC cells were detected by cell proliferation,wound-healing,and invasion assays,respectively.Results BCAT1 was highly expressed in cancer tissues of HNSC patients in the Cancer Genome Atlas(TCGA)database and was one of the independent prognostic fac-tors and potential diagnostic markers of patients.Results of cellular assays showed that BCAT1 expression was upregula-ted in human OSCC tissues or cell lines,thereby regulating the proliferation,migration,and invasion of OSCC cells.Con-clusion BCAT1 gene is a potential therapeutic target for human OSCC.关键词
支链氨基酸转氨酶1/口腔鳞状细胞癌/头颈鳞状细胞癌/增殖/迁移/侵袭Key words
branch-chain amino acid transaminase 1/oral squamous cell carcinoma/head and neck squamous cell carcinoma/proliferation/migration/invasion分类
临床医学引用本文复制引用
李熠洁,原振英,李明..支链氨基酸转氨酶1在口腔鳞状细胞癌中的表达及其功能研究[J].国际口腔医学杂志,2025,52(3):358-365,8.基金项目
Key Research and Development Project of Hunan Province(2022SK2052) 湖南省重点研发项目(2022SK2052) （2022SK2052）
